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Zinc-dependent regulation of zinc import and export genes by Zur

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  • Seung-Hwan Choi

    (Laboratory of Molecular Microbiology, School of Biological Sciences, and Institute of Microbiology, Seoul National University)

  • Kang-Lok Lee

    (Laboratory of Molecular Microbiology, School of Biological Sciences, and Institute of Microbiology, Seoul National University)

  • Jung-Ho Shin

    (Laboratory of Molecular Microbiology, School of Biological Sciences, and Institute of Microbiology, Seoul National University)

  • Yoo-Bok Cho

    (Laboratory of Molecular Microbiology, School of Biological Sciences, and Institute of Microbiology, Seoul National University)

  • Sun-Shin Cha

    (Ewha Womans University)

  • Jung-Hye Roe

    (Laboratory of Molecular Microbiology, School of Biological Sciences, and Institute of Microbiology, Seoul National University)

Abstract

In most bacteria, zinc depletion is sensed by Zur, whereas the surplus is sensed by different regulators to achieve zinc homeostasis. Here we present evidence that zinc-bound Zur not only represses genes for zinc acquisition but also induces the zitB gene encoding a zinc exporter in Streptomyces coelicolor, a model actinobacteria. Zinc-dependent gene regulation by Zur occurs in two phases. At sub-femtomolar zinc concentrations (phase I), dimeric Zur binds to the Zur-box motif immediately upstream of the zitB promoter, resulting in low zitB expression. At the same time, Zur represses genes for zinc uptake. At micromolar zinc concentrations (phase II), oligomeric Zur binding with footprint expansion upward from the Zur box results in high zitB induction. Our findings reveal a mode of zinc-dependent gene activation that uses a single metalloregulator to control genes for both uptake and export over a wide range of zinc concentrations.

Suggested Citation

  • Seung-Hwan Choi & Kang-Lok Lee & Jung-Ho Shin & Yoo-Bok Cho & Sun-Shin Cha & Jung-Hye Roe, 2017. "Zinc-dependent regulation of zinc import and export genes by Zur," Nature Communications, Nature, vol. 8(1), pages 1-11, August.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15812
    DOI: 10.1038/ncomms15812
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