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Single-peptide DNA-dependent RNA polymerase homologous to multi-subunit RNA polymerase

Author

Listed:
  • David Forrest

    (Centre for Bacterial Cell Biology, Institute for Cell and Molecular Bioscience, Newcastle University)

  • Katherine James

    (Centre for Bacterial Cell Biology, Institute for Cell and Molecular Bioscience, Newcastle University)

  • Yulia Yuzenkova

    (Centre for Bacterial Cell Biology, Institute for Cell and Molecular Bioscience, Newcastle University)

  • Nikolay Zenkin

    (Centre for Bacterial Cell Biology, Institute for Cell and Molecular Bioscience, Newcastle University)

Abstract

Transcription in all living organisms is accomplished by multi-subunit RNA polymerases (msRNAPs). msRNAPs are highly conserved in evolution and invariably share a ∼400 kDa five-subunit catalytic core. Here we characterize a hypothetical ∼100 kDa single-chain protein, YonO, encoded by the SPβ prophage of Bacillus subtilis. YonO shares very distant homology with msRNAPs, but no homology with single-subunit polymerases. We show that despite homology to only a few amino acids of msRNAP, and the absence of most of the conserved domains, YonO is a highly processive DNA-dependent RNA polymerase. We demonstrate that YonO is a bona fide RNAP of the SPβ bacteriophage that specifically transcribes its late genes, and thus represents a novel type of bacteriophage RNAPs. YonO and related proteins present in various bacteria and bacteriophages have diverged from msRNAPs before the Last Universal Common Ancestor, and, thus, may resemble the single-subunit ancestor of all msRNAPs.

Suggested Citation

  • David Forrest & Katherine James & Yulia Yuzenkova & Nikolay Zenkin, 2017. "Single-peptide DNA-dependent RNA polymerase homologous to multi-subunit RNA polymerase," Nature Communications, Nature, vol. 8(1), pages 1-8, August.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15774
    DOI: 10.1038/ncomms15774
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    Cited by:

    1. Anastasiia Chaban & Leonid Minakhin & Ekaterina Goldobina & Brain Bae & Yue Hao & Sergei Borukhov & Leena Putzeys & Maarten Boon & Florian Kabinger & Rob Lavigne & Kira S. Makarova & Eugene V. Koonin , 2024. "Tail-tape-fused virion and non-virion RNA polymerases of a thermophilic virus with an extremely long tail," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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