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Variation in auxin sensing guides AUX/IAA transcriptional repressor ubiquitylation and destruction

Author

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  • Martin Winkler

    (Leibniz Institute of Plant Biochemistry (IPB)
    Present address: Institute of Biochemistry, ETH Zurich, Zurich 8093, Switzerland)

  • Michael Niemeyer

    (Leibniz Institute of Plant Biochemistry (IPB))

  • Antje Hellmuth

    (Leibniz Institute of Plant Biochemistry (IPB))

  • Philipp Janitza

    (Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg)

  • Gideon Christ

    (Leibniz Institute of Plant Biochemistry (IPB))

  • Sophia L. Samodelov

    (Institute of Synthetic Biology, University of Düsseldorf
    Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg)

  • Verona Wilde

    (Leibniz Institute of Plant Biochemistry (IPB))

  • Petra Majovsky

    (Proteome Analytics Research Group, Leibniz Institute of Plant Biochemistry (IPB))

  • Marco Trujillo

    (Independent Junior Research Group Ubiquitination in Immunity, Leibniz Institute of Plant Biochemistry (IPB))

  • Matias D. Zurbriggen

    (Institute of Synthetic Biology, University of Düsseldorf
    Cluster of Excellence on Plant Science (CEPLAS), University of Düsseldorf)

  • Wolfgang Hoehenwarter

    (Proteome Analytics Research Group, Leibniz Institute of Plant Biochemistry (IPB))

  • Marcel Quint

    (Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg)

  • Luz Irina A. Calderón Villalobos

    (Leibniz Institute of Plant Biochemistry (IPB))

Abstract

Auxin is a small molecule morphogen that bridges SCFTIR1/AFB-AUX/IAA co-receptor interactions leading to ubiquitylation and proteasome-dependent degradation of AUX/IAA transcriptional repressors. Here, we systematically dissect auxin sensing by SCFTIR1-IAA6 and SCFTIR1-IAA19 co-receptor complexes, and assess IAA6/IAA19 ubiquitylation in vitro and IAA6/IAA19 degradation in vivo. We show that TIR1-IAA19 and TIR1-IAA6 have distinct auxin affinities that correlate with ubiquitylation and turnover dynamics of the AUX/IAA. We establish a system to track AUX/IAA ubiquitylation in IAA6 and IAA19 in vitro and show that it occurs in flexible hotspots in degron-flanking regions adorned with specific Lys residues. We propose that this signature is exploited during auxin-mediated SCFTIR1-AUX/IAA interactions. We present evidence for an evolving AUX/IAA repertoire, typified by the IAA6/IAA19 ohnologues, that discriminates the range of auxin concentrations found in plants. We postulate that the intrinsic flexibility of AUX/IAAs might bias their ubiquitylation and destruction kinetics enabling specific auxin responses.

Suggested Citation

  • Martin Winkler & Michael Niemeyer & Antje Hellmuth & Philipp Janitza & Gideon Christ & Sophia L. Samodelov & Verona Wilde & Petra Majovsky & Marco Trujillo & Matias D. Zurbriggen & Wolfgang Hoehenwart, 2017. "Variation in auxin sensing guides AUX/IAA transcriptional repressor ubiquitylation and destruction," Nature Communications, Nature, vol. 8(1), pages 1-13, August.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15706
    DOI: 10.1038/ncomms15706
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