IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_ncomms15692.html
   My bibliography  Save this article

Myosin Va molecular motors manoeuvre liposome cargo through suspended actin filament intersections in vitro

Author

Listed:
  • Andrew T. Lombardo

    (University of Vermont)

  • Shane R. Nelson

    (University of Vermont)

  • M. Yusuf Ali

    (University of Vermont)

  • Guy G. Kennedy

    (University of Vermont)

  • Kathleen M. Trybus

    (University of Vermont)

  • Sam Walcott

    (University of California)

  • David M. Warshaw

    (University of Vermont)

Abstract

Intracellular cargo transport relies on myosin Va molecular motor ensembles to travel along the cell’s three-dimensional (3D) highway of actin filaments. At actin filament intersections, the intersecting filament is a structural barrier to and an alternate track for directed cargo transport. Here we use 3D super-resolution fluorescence imaging to determine the directional outcome (that is, continues straight, turns or terminates) for an ∼10 motor ensemble transporting a 350 nm lipid-bound cargo that encounters a suspended 3D actin filament intersection in vitro. Motor–cargo complexes that interact with the intersecting filament go straight through the intersection 62% of the time, nearly twice that for turning. To explain this, we develop an in silico model, supported by optical trapping data, suggesting that the motors’ diffusive movements on the vesicle surface and the extent of their engagement with the two intersecting actin tracks biases the motor–cargo complex on average to go straight through the intersection.

Suggested Citation

  • Andrew T. Lombardo & Shane R. Nelson & M. Yusuf Ali & Guy G. Kennedy & Kathleen M. Trybus & Sam Walcott & David M. Warshaw, 2017. "Myosin Va molecular motors manoeuvre liposome cargo through suspended actin filament intersections in vitro," Nature Communications, Nature, vol. 8(1), pages 1-9, August.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15692
    DOI: 10.1038/ncomms15692
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms15692
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms15692?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Ashwin I. D’Souza & Rahul Grover & Gina A. Monzon & Ludger Santen & Stefan Diez, 2023. "Vesicles driven by dynein and kinesin exhibit directional reversals without regulators," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15692. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.