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MiCASA is a new method for quantifying cellular organization

Author

Listed:
  • Andrew Sornborger

    (University of California)

  • Jie Li

    (Paul D. Coverdell Center, University of Georgia)

  • Cullen Timmons

    (Paul D. Coverdell Center, University of Georgia
    Present address: Department of Emergency Medicine, Vanderbilt University Hospital, Nashville, Tennessee 37232, USA)

  • Floria Lupu

    (Paul D. Coverdell Center, University of Georgia)

  • Jonathan Eggenschwiler

    (Paul D. Coverdell Center, University of Georgia)

  • Yousuke Takahama

    (Institute for Genome Research, University of Tokushima)

  • Nancy R. Manley

    (Paul D. Coverdell Center, University of Georgia)

Abstract

While many tools exist for identifying and quantifying individual cell types, few methods are available to assess the relationships between cell types in organs and tissues and how these relationships change during aging or disease states. We present a quantitative method for evaluating cellular organization, using the mouse thymus as a test organ. The thymus is the primary lymphoid organ responsible for generating T cells in vertebrates, and its proper structure and organization is essential for optimal function. Our method, Multitaper Circularly Averaged Spectral Analysis (MiCASA), identifies differences in the tissue-level organization with high sensitivity, including defining a novel type of phenotype by measuring variability as a specific parameter. MiCASA provides a novel and easily implemented quantitative tool for assessing cellular organization.

Suggested Citation

  • Andrew Sornborger & Jie Li & Cullen Timmons & Floria Lupu & Jonathan Eggenschwiler & Yousuke Takahama & Nancy R. Manley, 2017. "MiCASA is a new method for quantifying cellular organization," Nature Communications, Nature, vol. 8(1), pages 1-10, August.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15619
    DOI: 10.1038/ncomms15619
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