IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_ncomms15576.html
   My bibliography  Save this article

Ebola virus VP30 and nucleoprotein interactions modulate viral RNA synthesis

Author

Listed:
  • Wei Xu

    (Washington University School of Medicine
    Present address: State Key Lab of Respiratory Disease, Guangzhou Eighth People’s Hospital, Guangzhou Medical University, Guangzhou 510060, China)

  • Priya Luthra

    (Center for Microbial Pathogenesis, Institute for Biomedical Sciences, Georgia State University)

  • Chao Wu

    (Washington University School of Medicine)

  • Jyoti Batra

    (Center for Microbial Pathogenesis, Institute for Biomedical Sciences, Georgia State University)

  • Daisy W. Leung

    (Washington University School of Medicine)

  • Christopher F. Basler

    (Center for Microbial Pathogenesis, Institute for Biomedical Sciences, Georgia State University)

  • Gaya K. Amarasinghe

    (Washington University School of Medicine)

Abstract

Ebola virus (EBOV) is an enveloped negative-sense RNA virus that causes sporadic outbreaks with high case fatality rates. Ebola viral protein 30 (eVP30) plays a critical role in EBOV transcription initiation at the nucleoprotein (eNP) gene, with additional roles in the replication cycle such as viral assembly. However, the mechanistic basis for how eVP30 functions during the virus replication cycle is currently unclear. Here we define a key interaction between eVP30 and a peptide derived from eNP that is important to facilitate interactions leading to the recognition of the RNA template. We present crystal structures of the eVP30 C-terminus in complex with this eNP peptide. Functional analyses of the eVP30–eNP interface identify residues that are critical for viral RNA synthesis. Altogether, these results support a model where the eVP30–eNP interaction plays a critical role in transcription initiation and provides a novel target for the development of antiviral therapy.

Suggested Citation

  • Wei Xu & Priya Luthra & Chao Wu & Jyoti Batra & Daisy W. Leung & Christopher F. Basler & Gaya K. Amarasinghe, 2017. "Ebola virus VP30 and nucleoprotein interactions modulate viral RNA synthesis," Nature Communications, Nature, vol. 8(1), pages 1-11, August.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15576
    DOI: 10.1038/ncomms15576
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms15576
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms15576?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Filip Mihalič & Caroline Benz & Eszter Kassa & Richard Lindqvist & Leandro Simonetti & Raviteja Inturi & Hanna Aronsson & Eva Andersson & Celestine N. Chi & Norman E. Davey & Anna K. Överby & Per Jemt, 2023. "Identification of motif-based interactions between SARS-CoV-2 protein domains and human peptide ligands pinpoint antiviral targets," Nature Communications, Nature, vol. 14(1), pages 1-18, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15576. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.