IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_ncomms15352.html
   My bibliography  Save this article

Activity-based protein profiling as a robust method for enzyme identification and screening in extremophilic Archaea

Author

Listed:
  • Susanne Zweerink

    (Chemische Biologie, ZMB, Fakultät für Biologie, Universität Duisburg-Essen)

  • Verena Kallnik

    (Molekulare Enzymtechnologie und Biochemie, Biofilm Centre, ZWU, Fakultät für Chemie, Universität Duisburg-Essen)

  • Sabrina Ninck

    (Chemische Biologie, ZMB, Fakultät für Biologie, Universität Duisburg-Essen)

  • Sabrina Nickel

    (Chemische Biologie, ZMB, Fakultät für Biologie, Universität Duisburg-Essen)

  • Julia Verheyen

    (Molekulare Enzymtechnologie und Biochemie, Biofilm Centre, ZWU, Fakultät für Chemie, Universität Duisburg-Essen)

  • Marcel Blum

    (Molekulare Enzymtechnologie und Biochemie, Biofilm Centre, ZWU, Fakultät für Chemie, Universität Duisburg-Essen)

  • Alexander Wagner

    (Molecular Biology of Archaea, Institute of Biology II—Microbiology, University of Freiburg)

  • Ingo Feldmann

    (Leibniz-Institut für Analytische Wissenschaften—ISAS e.V.)

  • Albert Sickmann

    (Leibniz-Institut für Analytische Wissenschaften—ISAS e.V.)

  • Sonja-Verena Albers

    (Molecular Biology of Archaea, Institute of Biology II—Microbiology, University of Freiburg)

  • Christopher Bräsen

    (Molekulare Enzymtechnologie und Biochemie, Biofilm Centre, ZWU, Fakultät für Chemie, Universität Duisburg-Essen)

  • Farnusch Kaschani

    (Chemische Biologie, ZMB, Fakultät für Biologie, Universität Duisburg-Essen)

  • Bettina Siebers

    (Molekulare Enzymtechnologie und Biochemie, Biofilm Centre, ZWU, Fakultät für Chemie, Universität Duisburg-Essen)

  • Markus Kaiser

    (Chemische Biologie, ZMB, Fakultät für Biologie, Universität Duisburg-Essen)

Abstract

Archaea are characterized by a unique life style in often environmental extremes but their thorough investigation is currently hampered by a limited set of suitable in vivo research methodologies. Here, we demonstrate that in vivo activity-based protein profiling (ABPP) may be used to sensitively detect either native or heterogeneously expressed active enzymes in living archaea even under these extreme conditions. In combination with the development of a genetically engineered archaeal screening strain, ABPP can furthermore be used in functional enzyme screenings from (meta)genome samples. We anticipate that our ABPP approach may therefore find application in basic archaeal research but also in the discovery of novel enzymes from (meta)genome libraries.

Suggested Citation

  • Susanne Zweerink & Verena Kallnik & Sabrina Ninck & Sabrina Nickel & Julia Verheyen & Marcel Blum & Alexander Wagner & Ingo Feldmann & Albert Sickmann & Sonja-Verena Albers & Christopher Bräsen & Farn, 2017. "Activity-based protein profiling as a robust method for enzyme identification and screening in extremophilic Archaea," Nature Communications, Nature, vol. 8(1), pages 1-12, August.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15352
    DOI: 10.1038/ncomms15352
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms15352
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms15352?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15352. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.