IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_ncomms15318.html
   My bibliography  Save this article

Live cell imaging of single genomic loci with quantum dot-labeled TALEs

Author

Listed:
  • Yingxin Ma

    (State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences
    Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology)

  • Mingxiu Wang

    (State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences)

  • Wei Li

    (State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences)

  • Zhiping Zhang

    (State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences)

  • Xiaowei Zhang

    (State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences)

  • Tianwei Tan

    (Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology)

  • Xian-En Zhang

    (CAS Center for Biological Macromolecules, National Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences)

  • Zongqiang Cui

    (State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences)

Abstract

Single genomic loci are often related to specific cellular functions, genetic diseases, or pathogenic infections. Visualization of single genomic loci in live human cells is currently of great interest, yet it remains challenging. Here, we describe a strategy for live cell imaging of single genomic loci by combining transcription activator-like effectors (TALEs) with a quantum dot labelling technique. We design and select a pair of TALEs that specifically target HIV-1 proviral DNA sequences, and use bioorthogonal ligation reactions to label them with different colour quantum dots (QDs). These QD-labelled TALEs are able to enter the cell nucleus to provide fluorescent signals to identify single gene loci. Based on the co-localization of the pair of different coloured QD-labelled TALEs, we determine and map single-copy HIV-1 provirus loci in human chromosomes in live host cells.

Suggested Citation

  • Yingxin Ma & Mingxiu Wang & Wei Li & Zhiping Zhang & Xiaowei Zhang & Tianwei Tan & Xian-En Zhang & Zongqiang Cui, 2017. "Live cell imaging of single genomic loci with quantum dot-labeled TALEs," Nature Communications, Nature, vol. 8(1), pages 1-8, August.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15318
    DOI: 10.1038/ncomms15318
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms15318
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms15318?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15318. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.