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The cohesin-like RecN protein stimulates RecA-mediated recombinational repair of DNA double-strand breaks

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  • Lee A. Uranga

    (New Mexico State University)

  • Emigdio D. Reyes

    (New Mexico State University)

  • Praveen L. Patidar

    (New Mexico State University)

  • Lindsay N. Redman

    (New Mexico State University)

  • Shelley L. Lusetti

    (New Mexico State University)

Abstract

RecN is a cohesin-like protein involved in DNA double-strand break repair in bacteria. The RecA recombinase functions to mediate repair via homologous DNA strand invasion to form D-loops. Here we provide evidence that the RecN protein stimulates the DNA strand invasion step of RecA-mediated recombinational DNA repair. The intermolecular DNA tethering activity of RecN protein described previously cannot fully explain this novel activity since stimulation of RecA function is species-specific and requires RecN ATP hydrolysis. Further, DNA-bound RecA protein increases the rate of ATP hydrolysis catalysed by RecN during the DNA pairing reaction. DNA-dependent RecN ATPase kinetics are affected by RecA protein in a manner suggesting a specific order of protein–DNA assembly, with RecN acting after RecA binds DNA. We present a model for RecN function that includes presynaptic stimulation of the bacterial repair pathway perhaps by contributing to the RecA homology search before ternary complex formation.

Suggested Citation

  • Lee A. Uranga & Emigdio D. Reyes & Praveen L. Patidar & Lindsay N. Redman & Shelley L. Lusetti, 2017. "The cohesin-like RecN protein stimulates RecA-mediated recombinational repair of DNA double-strand breaks," Nature Communications, Nature, vol. 8(1), pages 1-11, August.
  • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15282
    DOI: 10.1038/ncomms15282
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