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Protein-protein interactions and metabolite channelling in the plant tricarboxylic acid cycle

Author

Listed:
  • Youjun Zhang

    (Max-Planck-Institut für Molekulare Pflanzenphysiologie)

  • Katherine F. M. Beard

    (University of Oxford)

  • Corné Swart

    (Max-Planck-Institut für Molekulare Pflanzenphysiologie)

  • Susan Bergmann

    (Max-Planck-Institut für Molekulare Pflanzenphysiologie)

  • Ina Krahnert

    (Max-Planck-Institut für Molekulare Pflanzenphysiologie)

  • Zoran Nikoloski

    (Max-Planck-Institut für Molekulare Pflanzenphysiologie)

  • Alexander Graf

    (Max-Planck-Institut für Molekulare Pflanzenphysiologie)

  • R. George Ratcliffe

    (University of Oxford)

  • Lee J. Sweetlove

    (University of Oxford)

  • Alisdair R. Fernie

    (Max-Planck-Institut für Molekulare Pflanzenphysiologie)

  • Toshihiro Obata

    (Max-Planck-Institut für Molekulare Pflanzenphysiologie)

Abstract

Protein complexes of sequential metabolic enzymes, often termed metabolons, may permit direct channelling of metabolites between the enzymes, providing increased control over metabolic pathway fluxes. Experimental evidence supporting their existence in vivo remains fragmentary. In the present study, we test binary interactions of the proteins constituting the plant tricarboxylic acid (TCA) cycle. We integrate (semi-)quantitative results from affinity purification-mass spectrometry, split-luciferase and yeast-two-hybrid assays to generate a single reliability score for assessing protein–protein interactions. By this approach, we identify 158 interactions including those between catalytic subunits of sequential enzymes and between subunits of enzymes mediating non-adjacent reactions. We reveal channelling of citrate and fumarate in isolated potato mitochondria by isotope dilution experiments. These results provide evidence for a functional TCA cycle metabolon in plants, which we discuss in the context of contemporary understanding of this pathway in other kingdoms.

Suggested Citation

  • Youjun Zhang & Katherine F. M. Beard & Corné Swart & Susan Bergmann & Ina Krahnert & Zoran Nikoloski & Alexander Graf & R. George Ratcliffe & Lee J. Sweetlove & Alisdair R. Fernie & Toshihiro Obata, 2017. "Protein-protein interactions and metabolite channelling in the plant tricarboxylic acid cycle," Nature Communications, Nature, vol. 8(1), pages 1-11, August.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15212
    DOI: 10.1038/ncomms15212
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    Cited by:

    1. Weronika Jasinska & Mirco Dindo & Sandra M. C. Cordoba & Adrian W. R. Serohijos & Paola Laurino & Yariv Brotman & Shimon Bershtein, 2024. "Non-consecutive enzyme interactions within TCA cycle supramolecular assembly regulate carbon-nitrogen metabolism," Nature Communications, Nature, vol. 15(1), pages 1-15, December.

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