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An intermolecular FRET sensor detects the dynamics of T cell receptor clustering

Author

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  • Yuanqing Ma

    (EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales
    ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales)

  • Elvis Pandzic

    (EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales
    ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales)

  • Philip R. Nicovich

    (EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales
    ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales)

  • Yui Yamamoto

    (EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales
    ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales)

  • Joanna Kwiatek

    (EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales
    ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales)

  • Sophie V. Pageon

    (EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales
    ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales)

  • Aleš Benda

    (Biomedical Imaging Facility, Lowy Cancer Research Centre, University of New South Wales)

  • Jérémie Rossy

    (EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales
    ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales)

  • Katharina Gaus

    (EMBL Australia Node in Single Molecule Science, School of Medical Sciences, University of New South Wales
    ARC Centre of Excellence in Advanced Molecular Imaging, University of New South Wales)

Abstract

Clustering of the T-cell receptor (TCR) is thought to initiate downstream signalling. However, the detection of protein clustering with high spatial and temporal resolution remains challenging. Here we establish a Förster resonance energy transfer (FRET) sensor, named CliF, which reports intermolecular associations of neighbouring proteins in live cells. A key advantage of the single-chain FRET sensor is that it can be combined with image correlation spectroscopy (ICS), single-particle tracking (SPT) and fluorescence lifetime imaging microscopy (FLIM). We test the sensor with a light-sensitive actuator that induces protein aggregation upon radiation with blue light. When applied to T cells, the sensor reveals that TCR triggering increases the number of dense TCR–CD3 clusters. Further, we find a correlation between cluster movement within the immunological synapse and cluster density. In conclusion, we develop a sensor that allows us to map the dynamics of protein clustering in live T cells.

Suggested Citation

  • Yuanqing Ma & Elvis Pandzic & Philip R. Nicovich & Yui Yamamoto & Joanna Kwiatek & Sophie V. Pageon & Aleš Benda & Jérémie Rossy & Katharina Gaus, 2017. "An intermolecular FRET sensor detects the dynamics of T cell receptor clustering," Nature Communications, Nature, vol. 8(1), pages 1-11, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15100
    DOI: 10.1038/ncomms15100
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    Cited by:

    1. Darren B. McAffee & Mark K. O’Dair & Jenny J. Lin & Shalini T. Low-Nam & Kiera B. Wilhelm & Sungi Kim & Shumpei Morita & Jay T. Groves, 2022. "Discrete LAT condensates encode antigen information from single pMHC:TCR binding events," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    2. Sorosh Amiri & Camelia Muresan & Xingbo Shang & Clotilde Huet-Calderwood & Martin A. Schwartz & David A. Calderwood & Michael Murrell, 2023. "Intracellular tension sensor reveals mechanical anisotropy of the actin cytoskeleton," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

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