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The molecular mechanism of the type IVa pilus motors

Author

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  • Matthew McCallum

    (University of Toronto
    Program in Molecular Medicine, Peter Gilgan Centre for Research and Learning, The Hospital for Sick Children)

  • Stephanie Tammam

    (Program in Molecular Medicine, Peter Gilgan Centre for Research and Learning, The Hospital for Sick Children)

  • Ahmad Khan

    (University of Toronto)

  • Lori L. Burrows

    (McMaster University)

  • P. Lynne Howell

    (University of Toronto
    Program in Molecular Medicine, Peter Gilgan Centre for Research and Learning, The Hospital for Sick Children)

Abstract

Type IVa pili are protein filaments essential for virulence in many bacterial pathogens; they extend and retract from the surface of bacterial cells to pull the bacteria forward. The motor ATPase PilB powers pilus assembly. Here we report the structures of the core ATPase domains of Geobacter metallireducens PilB bound to ADP and the non-hydrolysable ATP analogue, AMP-PNP, at 3.4 and 2.3 Å resolution, respectively. These structures reveal important differences in nucleotide binding between chains. Analysis of these differences reveals the sequential turnover of nucleotide, and the corresponding domain movements. Our data suggest a clockwise rotation of the central sub-pores of PilB, which through interactions with PilC, would support the assembly of a right-handed helical pilus. Our analysis also suggests a counterclockwise rotation of the C2 symmetric PilT that would enable right-handed pilus disassembly. The proposed model provides insight into how this family of ATPases can power pilus extension and retraction.

Suggested Citation

  • Matthew McCallum & Stephanie Tammam & Ahmad Khan & Lori L. Burrows & P. Lynne Howell, 2017. "The molecular mechanism of the type IVa pilus motors," Nature Communications, Nature, vol. 8(1), pages 1-10, August.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15091
    DOI: 10.1038/ncomms15091
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    Cited by:

    1. Michael Hohl & Emma J. Banks & Max P. Manley & Tung B. K. Le & Harry H. Low, 2024. "Bidirectional pilus processing in the Tad pilus system motor CpaF," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    2. Shuaiqi Guo & Yunjie Chang & Yves V. Brun & P. Lynne Howell & Lori L. Burrows & Jun Liu, 2024. "PilY1 regulates the dynamic architecture of the type IV pilus machine in Pseudomonas aeruginosa," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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