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The conserved protein Seb1 drives transcription termination by binding RNA polymerase II and nascent RNA

Author

Listed:
  • Sina Wittmann

    (University of Oxford)

  • Max Renner

    (Wellcome Trust Centre for Human Genetics, University of Oxford)

  • Beth R. Watts

    (University of Oxford)

  • Oliver Adams

    (University of Oxford)

  • Miles Huseyin

    (University of Oxford)

  • Carlo Baejen

    (Max Planck Institute for Biophysical Chemistry)

  • Kamel El Omari

    (Diamond Light Source Ltd, Harwell Science & Innovation Campus)

  • Cornelia Kilchert

    (University of Oxford)

  • Dong-Hyuk Heo

    (University of Oxford)

  • Tea Kecman

    (University of Oxford)

  • Patrick Cramer

    (Max Planck Institute for Biophysical Chemistry)

  • Jonathan M. Grimes

    (Wellcome Trust Centre for Human Genetics, University of Oxford
    Diamond Light Source Ltd, Harwell Science & Innovation Campus)

  • Lidia Vasiljeva

    (University of Oxford)

Abstract

Termination of RNA polymerase II (Pol II) transcription is an important step in the transcription cycle, which involves the dislodgement of polymerase from DNA, leading to release of a functional transcript. Recent studies have identified the key players required for this process and showed that a common feature of these proteins is a conserved domain that interacts with the phosphorylated C-terminus of Pol II (CTD-interacting domain, CID). However, the mechanism by which transcription termination is achieved is not understood. Using genome-wide methods, here we show that the fission yeast CID-protein Seb1 is essential for termination of protein-coding and non-coding genes through interaction with S2-phosphorylated Pol II and nascent RNA. Furthermore, we present the crystal structures of the Seb1 CTD- and RNA-binding modules. Unexpectedly, the latter reveals an intertwined two-domain arrangement of a canonical RRM and second domain. These results provide important insights into the mechanism underlying eukaryotic transcription termination.

Suggested Citation

  • Sina Wittmann & Max Renner & Beth R. Watts & Oliver Adams & Miles Huseyin & Carlo Baejen & Kamel El Omari & Cornelia Kilchert & Dong-Hyuk Heo & Tea Kecman & Patrick Cramer & Jonathan M. Grimes & Lidia, 2017. "The conserved protein Seb1 drives transcription termination by binding RNA polymerase II and nascent RNA," Nature Communications, Nature, vol. 8(1), pages 1-15, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14861
    DOI: 10.1038/ncomms14861
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    Cited by:

    1. Ebru Aydin & Silke Schreiner & Jacqueline Böhme & Birte Keil & Jan Weber & Bojan Žunar & Timo Glatter & Cornelia Kilchert, 2024. "DEAD-box ATPase Dbp2 is the key enzyme in an mRNP assembly checkpoint at the 3’-end of genes and involved in the recycling of cleavage factors," Nature Communications, Nature, vol. 15(1), pages 1-20, December.
    2. Maxime Duval & Carlo Yague-Sanz & Tomasz W. Turowski & Elisabeth Petfalski & David Tollervey & François Bachand, 2023. "The conserved RNA-binding protein Seb1 promotes cotranscriptional ribosomal RNA processing by controlling RNA polymerase I progression," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

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