IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_ncomms14813.html
   My bibliography  Save this article

Cell-free reconstitution reveals centriole cartwheel assembly mechanisms

Author

Listed:
  • P. Guichard

    (Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL)
    Present address: Department of Cell Biology, Sciences III, University of Geneva, Geneva CH-1211, Switzerland)

  • V. Hamel

    (Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL)
    Present address: Department of Cell Biology, Sciences III, University of Geneva, Geneva CH-1211, Switzerland)

  • M. Le Guennec

    (Sciences III, University of Geneva)

  • N. Banterle

    (Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL))

  • I. Iacovache

    (Institute of Anatomy, University of Bern)

  • V. Nemčíková

    (Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL))

  • I. Flückiger

    (Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL))

  • K. N. Goldie

    (Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel)

  • H. Stahlberg

    (Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel)

  • D. Lévy

    (Institut Curie, PSL Research University, UMR 168, Centre de Recherche, 26 rue d’ULM, Paris 75231, France)

  • B. Zuber

    (Institute of Anatomy, University of Bern)

  • P. Gönczy

    (Swiss Institute for Experimental Cancer Research (ISREC), School of Life Sciences, Swiss Federal Institute of Technology (EPFL))

Abstract

How cellular organelles assemble is a fundamental question in biology. The centriole organelle organizes around a nine-fold symmetrical cartwheel structure typically ∼100 nm high comprising a stack of rings that each accommodates nine homodimers of SAS-6 proteins. Whether nine-fold symmetrical ring-like assemblies of SAS-6 proteins harbour more peripheral cartwheel elements is unclear. Furthermore, the mechanisms governing ring stacking are not known. Here we develop a cell-free reconstitution system for core cartwheel assembly. Using cryo-electron tomography, we uncover that the Chlamydomonas reinhardtii proteins CrSAS-6 and Bld10p together drive assembly of the core cartwheel. Moreover, we discover that CrSAS-6 possesses autonomous properties that ensure self-organized ring stacking. Mathematical fitting of reconstituted cartwheel height distribution suggests a mechanism whereby preferential addition of pairs of SAS-6 rings governs cartwheel growth. In conclusion, we have developed a cell-free reconstitution system that reveals fundamental assembly principles at the root of centriole biogenesis.

Suggested Citation

  • P. Guichard & V. Hamel & M. Le Guennec & N. Banterle & I. Iacovache & V. Nemčíková & I. Flückiger & K. N. Goldie & H. Stahlberg & D. Lévy & B. Zuber & P. Gönczy, 2017. "Cell-free reconstitution reveals centriole cartwheel assembly mechanisms," Nature Communications, Nature, vol. 8(1), pages 1-9, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14813
    DOI: 10.1038/ncomms14813
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms14813
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms14813?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Niccolò Banterle & Adrian P. Nievergelt & Svenja Buhr & Georgios N. Hatzopoulos & Charlène Brillard & Santiago Andany & Tania Hübscher & Frieda A. Sorgenfrei & Ulrich S. Schwarz & Frauke Gräter & Geor, 2021. "Kinetic and structural roles for the surface in guiding SAS-6 self-assembly to direct centriole architecture," Nature Communications, Nature, vol. 12(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14813. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.