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Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots

Author

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  • Eugene A. Katrukha

    (Cell Biology, Faculty of Science, Utrecht University)

  • Marina Mikhaylova

    (Cell Biology, Faculty of Science, Utrecht University
    RG Neuroplasticity, Leibniz Institute for Neurobiology
    Present address: University Medical Center Hamburg-Eppendorf, UKE, Center for Molecular Neurobiology, ZMNH, Falkenried 94, 20251 Hamburg, Germany)

  • Hugo X. van Brakel

    (Cell Biology, Faculty of Science, Utrecht University)

  • Paul M. van Bergen en Henegouwen

    (Cell Biology, Faculty of Science, Utrecht University)

  • Anna Akhmanova

    (Cell Biology, Faculty of Science, Utrecht University)

  • Casper C. Hoogenraad

    (Cell Biology, Faculty of Science, Utrecht University)

  • Lukas C. Kapitein

    (Cell Biology, Faculty of Science, Utrecht University)

Abstract

The cytoplasm is a highly complex and heterogeneous medium that is structured by the cytoskeleton. How local transport depends on the heterogeneous organization and dynamics of F-actin and microtubules is poorly understood. Here we use a novel delivery and functionalization strategy to utilize quantum dots (QDs) as probes for active and passive intracellular transport. Rapid imaging of non-functionalized QDs reveals two populations with a 100-fold difference in diffusion constant, with the faster fraction increasing upon actin depolymerization. When nanobody-functionalized QDs are targeted to different kinesin motor proteins, their trajectories do not display strong actin-induced transverse displacements, as suggested previously. Only kinesin-1 displays subtle directional fluctuations, because the subset of microtubules used by this motor undergoes prominent undulations. Using actin-targeting agents reveals that F-actin suppresses most microtubule shape remodelling, rather than promoting it. These results demonstrate how the spatial heterogeneity of the cytoskeleton imposes large variations in non-equilibrium intracellular dynamics.

Suggested Citation

  • Eugene A. Katrukha & Marina Mikhaylova & Hugo X. van Brakel & Paul M. van Bergen en Henegouwen & Anna Akhmanova & Casper C. Hoogenraad & Lukas C. Kapitein, 2017. "Probing cytoskeletal modulation of passive and active intracellular dynamics using nanobody-functionalized quantum dots," Nature Communications, Nature, vol. 8(1), pages 1-8, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14772
    DOI: 10.1038/ncomms14772
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    Cited by:

    1. William Y. C. Huang & Xianrui Cheng & James E. Ferrell, 2022. "Cytoplasmic organization promotes protein diffusion in Xenopus extracts," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    2. Sikora, Grzegorz & Wyłomańska, Agnieszka & Krapf, Diego, 2018. "Recurrence statistics for anomalous diffusion regime change detection," Computational Statistics & Data Analysis, Elsevier, vol. 128(C), pages 380-394.

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