Author
Listed:
- Marie Trussart
(EMBL/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
Universitat Pompeu Fabra (UPF))
- Eva Yus
(EMBL/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
Universitat Pompeu Fabra (UPF))
- Sira Martinez
(EMBL/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology)
- Davide Baù
(Gene Regulation, Stem Cells and Cancer Program. Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
CNAG-CRG, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology)
- Yuhei O. Tahara
(Graduate School of Science, Osaka City University
OCU Advanced Research Institute for Natural Science and Technology (OCARNA), Osaka City University)
- Thomas Pengo
(EMBL/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
Advanced Light Microscopy Unit, Centre for Genomic Regulation (CRG))
- Michael Widjaja
(The ithree Institute, The University of Technology Sydney, Sydney, New South Wales 2007, Australia)
- Simon Kretschmer
(Max Planck Institute of Biochemistry)
- Jim Swoger
(EMBL/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
Universitat Pompeu Fabra (UPF))
- Steven Djordjevic
(The ithree Institute, The University of Technology Sydney, Sydney, New South Wales 2007, Australia)
- Lynne Turnbull
(The ithree Institute, The University of Technology Sydney, Sydney, New South Wales 2007, Australia)
- Cynthia Whitchurch
(The ithree Institute, The University of Technology Sydney, Sydney, New South Wales 2007, Australia)
- Makoto Miyata
(Graduate School of Science, Osaka City University
OCU Advanced Research Institute for Natural Science and Technology (OCARNA), Osaka City University)
- Marc A. Marti-Renom
(Universitat Pompeu Fabra (UPF)
Gene Regulation, Stem Cells and Cancer Program. Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
CNAG-CRG, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
Institució Catalana de Recerca i Estudis Avançats (ICREA))
- Maria Lluch-Senar
(EMBL/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
Universitat Pompeu Fabra (UPF))
- Luís Serrano
(EMBL/CRG Systems Biology Research Unit, Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology
Universitat Pompeu Fabra (UPF)
Institució Catalana de Recerca i Estudis Avançats (ICREA))
Abstract
DNA-binding proteins are central regulators of chromosome organization; however, in genome-reduced bacteria their diversity is largely diminished. Whether the chromosomes of such bacteria adopt defined three-dimensional structures remains unexplored. Here we combine Hi-C and super-resolution microscopy to determine the structure of the Mycoplasma pneumoniae chromosome at a 10 kb resolution. We find a defined structure, with a global symmetry between two arms that connect opposite poles, one bearing the chromosomal Ori and the other the midpoint. Analysis of local structures at a 3 kb resolution indicates that the chromosome is organized into domains ranging from 15 to 33 kb. We provide evidence that genes within the same domain tend to be co-regulated, suggesting that chromosome organization influences transcriptional regulation, and that supercoiling regulates local organization. This study extends the current understanding of bacterial genome organization and demonstrates that a defined chromosomal structure is a universal feature of living systems.
Suggested Citation
Marie Trussart & Eva Yus & Sira Martinez & Davide Baù & Yuhei O. Tahara & Thomas Pengo & Michael Widjaja & Simon Kretschmer & Jim Swoger & Steven Djordjevic & Lynne Turnbull & Cynthia Whitchurch & Mak, 2017.
"Defined chromosome structure in the genome-reduced bacterium Mycoplasma pneumoniae,"
Nature Communications, Nature, vol. 8(1), pages 1-13, April.
Handle:
RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14665
DOI: 10.1038/ncomms14665
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Citations
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Cited by:
- Alicia Broto & Erika Gaspari & Samuel Miravet-Verde & Vitor A. P. Martins Santos & Mark Isalan, 2022.
"A genetic toolkit and gene switches to limit Mycoplasma growth for biosafety applications,"
Nature Communications, Nature, vol. 13(1), pages 1-13, December.
- Miloš Tišma & Florian Patrick Bock & Jacob Kerssemakers & Hammam Antar & Aleksandre Japaridze & Stephan Gruber & Cees Dekker, 2024.
"Direct observation of a crescent-shape chromosome in expanded Bacillus subtilis cells,"
Nature Communications, Nature, vol. 15(1), pages 1-13, December.
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