IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_ncomms14648.html
   My bibliography  Save this article

The SWI/SNF chromatin remodelling complex is required for maintenance of lineage specific enhancers

Author

Listed:
  • Burak H. Alver

    (Harvard Medical School)

  • Kimberly H. Kim

    (Dana-Farber Cancer Institute
    Children's Hospital
    Harvard Medical School
    Present address: Department of Oncology Target Discovery, Pfizer, Pearl River, New York 10965, USA)

  • Ping Lu

    (Dana-Farber Cancer Institute
    Children's Hospital
    Harvard Medical School)

  • Xiaofeng Wang

    (Dana-Farber Cancer Institute
    Children's Hospital
    Harvard Medical School)

  • Haley E. Manchester

    (Dana-Farber Cancer Institute
    Children's Hospital
    Harvard Medical School)

  • Weishan Wang

    (Dana-Farber Cancer Institute
    Children's Hospital
    Harvard Medical School)

  • Jeffrey R. Haswell

    (Dana-Farber Cancer Institute
    Children's Hospital
    Harvard Medical School)

  • Peter J. Park

    (Harvard Medical School)

  • Charles W. M. Roberts

    (Dana-Farber Cancer Institute
    Children's Hospital
    Harvard Medical School
    St. Jude Children's Research Hospital)

Abstract

Genes encoding subunits of SWI/SNF (BAF) chromatin remodelling complexes are collectively altered in over 20% of human malignancies, but the mechanisms by which these complexes alter chromatin to modulate transcription and cell fate are poorly understood. Utilizing mouse embryonic fibroblast and cancer cell line models, here we show via ChIP-seq and biochemical assays that SWI/SNF complexes are preferentially targeted to distal lineage specific enhancers and interact with p300 to modulate histone H3 lysine 27 acetylation. We identify a greater requirement for SWI/SNF at typical enhancers than at most super-enhancers and at enhancers in untranscribed regions than in transcribed regions. Our data further demonstrate that SWI/SNF-dependent distal enhancers are essential for controlling expression of genes linked to developmental processes. Our findings thus establish SWI/SNF complexes as regulators of the enhancer landscape and provide insight into the roles of SWI/SNF in cellular fate control.

Suggested Citation

  • Burak H. Alver & Kimberly H. Kim & Ping Lu & Xiaofeng Wang & Haley E. Manchester & Weishan Wang & Jeffrey R. Haswell & Peter J. Park & Charles W. M. Roberts, 2017. "The SWI/SNF chromatin remodelling complex is required for maintenance of lineage specific enhancers," Nature Communications, Nature, vol. 8(1), pages 1-10, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14648
    DOI: 10.1038/ncomms14648
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms14648
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms14648?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Cheng Zeng & Jiwei Chen & Emmalee W. Cooke & Arijita Subuddhi & Eliana T. Roodman & Fei Xavier Chen & Kaixiang Cao, 2023. "Demethylase-independent roles of LSD1 in regulating enhancers and cell fate transition," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    2. Jan-Renier Moonen & James Chappell & Minyi Shi & Tsutomu Shinohara & Dan Li & Maxwell R. Mumbach & Fan Zhang & Ramesh V. Nair & Joseph Nasser & Daniel H. Mai & Shalina Taylor & Lingli Wang & Ross J. M, 2022. "KLF4 recruits SWI/SNF to increase chromatin accessibility and reprogram the endothelial enhancer landscape under laminar shear stress," Nature Communications, Nature, vol. 13(1), pages 1-16, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14648. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.