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Profiling protein expression in circulating tumour cells using microfluidic western blotting

Author

Listed:
  • Elly Sinkala

    (University of California, Berkeley)

  • Elodie Sollier-Christen

    (Vortex Biosciences, Inc.
    Stanford University School of Medicine)

  • Corinne Renier

    (Vortex Biosciences, Inc.
    Stanford University School of Medicine)

  • Elisabet Rosàs-Canyelles

    (University of California, Berkeley
    The UC Berkeley–UCSF Graduate Program in Bioengineering, University of California)

  • James Che

    (Vortex Biosciences, Inc.
    University of California, Los Angeles)

  • Kyra Heirich

    (Stanford University School of Medicine)

  • Todd A. Duncombe

    (University of California, Berkeley
    The UC Berkeley–UCSF Graduate Program in Bioengineering, University of California)

  • Julea Vlassakis

    (University of California, Berkeley
    The UC Berkeley–UCSF Graduate Program in Bioengineering, University of California)

  • Kevin A. Yamauchi

    (University of California, Berkeley
    The UC Berkeley–UCSF Graduate Program in Bioengineering, University of California)

  • Haiyan Huang

    (University of California)

  • Stefanie S. Jeffrey

    (Stanford University School of Medicine)

  • Amy E. Herr

    (University of California, Berkeley
    The UC Berkeley–UCSF Graduate Program in Bioengineering, University of California)

Abstract

Circulating tumour cells (CTCs) are rare tumour cells found in the circulatory system of certain cancer patients. The clinical and functional significance of CTCs is still under investigation. Protein profiling of CTCs would complement the recent advances in enumeration, transcriptomic and genomic characterization of these rare cells and help define their characteristics. Here we describe a microfluidic western blot for an eight-plex protein panel for individual CTCs derived from estrogen receptor-positive (ER+) breast cancer patients. The precision handling and analysis reveals a capacity to assay sparingly available patient-derived CTCs, a biophysical CTC phenotype more lysis-resistant than breast cancer cell lines, a capacity to report protein expression on a per CTC basis and two statistically distinct GAPDH subpopulations within the patient-derived CTCs. Targeted single-CTC proteomics with the capacity for archivable, multiplexed protein analysis offers a unique, complementary taxonomy for understanding CTC biology and ascertaining clinical impact.

Suggested Citation

  • Elly Sinkala & Elodie Sollier-Christen & Corinne Renier & Elisabet Rosàs-Canyelles & James Che & Kyra Heirich & Todd A. Duncombe & Julea Vlassakis & Kevin A. Yamauchi & Haiyan Huang & Stefanie S. Jeff, 2017. "Profiling protein expression in circulating tumour cells using microfluidic western blotting," Nature Communications, Nature, vol. 8(1), pages 1-12, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14622
    DOI: 10.1038/ncomms14622
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    Cited by:

    1. Xiaoxu Guo & Fanghe Lin & Chuanyou Yi & Juan Song & Di Sun & Li Lin & Zhixing Zhong & Zhaorun Wu & Xiaoyu Wang & Yingkun Zhang & Jin Li & Huimin Zhang & Feng Liu & Chaoyong Yang & Jia Song, 2022. "Deep transfer learning enables lesion tracing of circulating tumor cells," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    2. Sofani Tafesse Gebreyesus & Asad Ali Siyal & Reta Birhanu Kitata & Eric Sheng-Wen Chen & Bayarmaa Enkhbayar & Takashi Angata & Kuo-I Lin & Yu-Ju Chen & Hsiung-Lin Tu, 2022. "Streamlined single-cell proteomics by an integrated microfluidic chip and data-independent acquisition mass spectrometry," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

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