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BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis

Author

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  • Wenbo Liu

    (School of Life Sciences, University of Science and Technology of China
    State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences)

  • Fengchao Wang

    (National Institute of Biological Sciences)

  • Qianhua Xu

    (State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences)

  • Junchao Shi

    (State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences)

  • Xiaoxin Zhang

    (State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences)

  • Xukun Lu

    (State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences)

  • Zhen-Ao Zhao

    (State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences)

  • Zheng Gao

    (State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences)

  • Huaixiao Ma

    (State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences)

  • Enkui Duan

    (State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Fei Gao

    (State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Shaorong Gao

    (School of Life Sciences and Technology, Tongji University)

  • Zhaohong Yi

    (Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture)

  • Lei Li

    (State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

Abstract

Breast cancer amplified sequence 2 (BCAS2) is involved in multiple biological processes, including pre-mRNA splicing. However, the physiological roles of BCAS2 are still largely unclear. Here we report that BCAS2 is specifically enriched in spermatogonia of mouse testes. Conditional disruption of Bcas2 in male germ cells impairs spermatogenesis and leads to male mouse infertility. Although the spermatogonia appear grossly normal, spermatocytes in meiosis prophase I and meiosis events (recombination and synapsis) are rarely observed in the BCAS2-depleted testis. In BCAS2 null testis, 245 genes are altered in alternative splicing forms; at least three spermatogenesis-related genes (Dazl, Ehmt2 and Hmga1) can be verified. In addition, disruption of Bcas2 results in a significant decrease of the full-length form and an increase of the short form (lacking exon 8) of DAZL protein. Altogether, our results suggest that BCAS2 regulates alternative splicing in spermatogonia and the transition to meiosis initiation, and male fertility.

Suggested Citation

  • Wenbo Liu & Fengchao Wang & Qianhua Xu & Junchao Shi & Xiaoxin Zhang & Xukun Lu & Zhen-Ao Zhao & Zheng Gao & Huaixiao Ma & Enkui Duan & Fei Gao & Shaorong Gao & Zhaohong Yi & Lei Li, 2017. "BCAS2 is involved in alternative mRNA splicing in spermatogonia and the transition to meiosis," Nature Communications, Nature, vol. 8(1), pages 1-11, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14182
    DOI: 10.1038/ncomms14182
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    Cited by:

    1. Shenglei Feng & Jinmei Li & Hui Wen & Kuan Liu & Yiqian Gui & Yujiao Wen & Xiaoli Wang & Shuiqiao Yuan, 2022. "hnRNPH1 recruits PTBP2 and SRSF3 to modulate alternative splicing in germ cells," Nature Communications, Nature, vol. 13(1), pages 1-19, December.

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