Author
Listed:
- Jonas Doerr
(Institute of Reconstructive Neurobiology, University of Bonn
Life&Brain GmbH)
- Martin Karl Schwarz
(Life&Brain GmbH
Functional Neuroconnectomics Group, University of Bonn)
- Dirk Wiedermann
(Max Planck Institute for Metabolism Research, In-vivo-NMR Laboratory)
- Anke Leinhaas
(Institute of Reconstructive Neurobiology, University of Bonn)
- Alina Jakobs
(Institute of Reconstructive Neurobiology, University of Bonn)
- Florian Schloen
(Institute of Physical and Theoretical Chemistry, University of Bonn)
- Inna Schwarz
(Functional Neuroconnectomics Group, University of Bonn)
- Michael Diedenhofen
(Max Planck Institute for Metabolism Research, In-vivo-NMR Laboratory)
- Nils Christian Braun
(Institute of Reconstructive Neurobiology, University of Bonn)
- Philipp Koch
(Institute of Reconstructive Neurobiology, University of Bonn)
- Daniel A. Peterson
(Center for Stem Cell and Regenerative Medicine, Rosalind Franklin University of Medicine and Science)
- Ulrich Kubitscheck
(Institute of Physical and Theoretical Chemistry, University of Bonn)
- Mathias Hoehn
(Max Planck Institute for Metabolism Research, In-vivo-NMR Laboratory)
- Oliver Brüstle
(Institute of Reconstructive Neurobiology, University of Bonn
Life&Brain GmbH)
Abstract
While transplantation represents a key tool for assessing in vivo functionality of neural stem cells and their suitability for neural repair, little is known about the integration of grafted neurons into the host brain circuitry. Rabies virus-based retrograde tracing has developed into a powerful approach for visualizing synaptically connected neurons. Here, we combine this technique with light sheet fluorescence microscopy (LSFM) to visualize transplanted cells and connected host neurons in whole-mouse brain preparations. Combined with co-registration of high-precision three-dimensional magnetic resonance imaging (3D MRI) reference data sets, this approach enables precise anatomical allocation of the host input neurons. Our data show that the same neural donor cell population grafted into different brain regions receives highly orthotopic input. These findings indicate that transplant connectivity is largely dictated by the circuitry of the target region and depict rabies-based transsynaptic tracing and LSFM as efficient tools for comprehensive assessment of host–donor cell innervation.
Suggested Citation
Jonas Doerr & Martin Karl Schwarz & Dirk Wiedermann & Anke Leinhaas & Alina Jakobs & Florian Schloen & Inna Schwarz & Michael Diedenhofen & Nils Christian Braun & Philipp Koch & Daniel A. Peterson & U, 2017.
"Whole-brain 3D mapping of human neural transplant innervation,"
Nature Communications, Nature, vol. 8(1), pages 1-7, April.
Handle:
RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14162
DOI: 10.1038/ncomms14162
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