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Repo-Man/PP1 regulates heterochromatin formation in interphase

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Listed:
  • Inês J. de Castro

    (College of Health and Life Science, Research Institute for Environment Health and Society, Brunel University London)

  • James Budzak

    (College of Health and Life Science, Research Institute for Environment Health and Society, Brunel University London
    Present address: Division of Cell and Molecular Biology, Department of Life Sciences, Imperial College London, London SW7 2AZ, UK)

  • Maria L. Di Giacinto

    (College of Health and Life Science, Research Institute for Environment Health and Society, Brunel University London)

  • Lorena Ligammari

    (College of Health and Life Science, Research Institute for Environment Health and Society, Brunel University London)

  • Ezgi Gokhan

    (College of Health and Life Science, Research Institute for Environment Health and Society, Brunel University London)

  • Christos Spanos

    (Wellcome Trust Centre for Cell Biology)

  • Daniela Moralli

    (Wellcome Trust Centre for Human Genetics, University of Oxford)

  • Christine Richardson

    (School of Biological and Medical Science, Durham University)

  • Jose I. de las Heras

    (Wellcome Trust Centre for Cell Biology)

  • Silvia Salatino

    (Wellcome Trust Centre for Human Genetics, University of Oxford)

  • Eric C. Schirmer

    (Wellcome Trust Centre for Cell Biology)

  • Katharine S. Ullman

    (Huntsman Cancer Institute, University of Utah)

  • Wendy A. Bickmore

    (MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh)

  • Catherine Green

    (Wellcome Trust Centre for Human Genetics, University of Oxford)

  • Juri Rappsilber

    (Wellcome Trust Centre for Cell Biology
    Technische Universitat Berlin)

  • Sarah Lamble

    (Wellcome Trust Centre for Human Genetics, University of Oxford)

  • Martin W. Goldberg

    (School of Biological and Medical Science, Durham University)

  • Veronica Vinciotti

    (College of Engineering, Design and Technology, Research Institute for Environment Health and Society, Brunel University London)

  • Paola Vagnarelli

    (College of Health and Life Science, Research Institute for Environment Health and Society, Brunel University London)

Abstract

Repo-Man is a protein phosphatase 1 (PP1) targeting subunit that regulates mitotic progression and chromatin remodelling. After mitosis, Repo-Man/PP1 remains associated with chromatin but its function in interphase is not known. Here we show that Repo-Man, via Nup153, is enriched on condensed chromatin at the nuclear periphery and at the edge of the nucleopore basket. Repo-Man/PP1 regulates the formation of heterochromatin, dephosphorylates H3S28 and it is necessary and sufficient for heterochromatin protein 1 binding and H3K27me3 recruitment. Using a novel proteogenomic approach, we show that Repo-Man is enriched at subtelomeric regions together with H2AZ and H3.3 and that depletion of Repo-Man alters the peripheral localization of a subset of these regions and alleviates repression of some polycomb telomeric genes. This study shows a role for a mitotic phosphatase in the regulation of the epigenetic landscape and gene expression in interphase.

Suggested Citation

  • Inês J. de Castro & James Budzak & Maria L. Di Giacinto & Lorena Ligammari & Ezgi Gokhan & Christos Spanos & Daniela Moralli & Christine Richardson & Jose I. de las Heras & Silvia Salatino & Eric C. S, 2017. "Repo-Man/PP1 regulates heterochromatin formation in interphase," Nature Communications, Nature, vol. 8(1), pages 1-16, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14048
    DOI: 10.1038/ncomms14048
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    Cited by:

    1. Shelby Winans & Hyun Jae Yu & Kenia los Santos & Gary Z. Wang & Vineet N. KewalRamani & Stephen P. Goff, 2022. "A point mutation in HIV-1 integrase redirects proviral integration into centromeric repeats," Nature Communications, Nature, vol. 13(1), pages 1-12, December.

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