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Structural and mechanistic basis of proton-coupled metal ion transport in the SLC11/NRAMP family

Author

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  • Ines A. Ehrnstorfer

    (University of Zurich)

  • Cristina Manatschal

    (University of Zurich)

  • Fabian M. Arnold

    (University of Zurich
    Present address: Institute of Medical Microbiology, Gloriastrasse 30/32, University of Zurich, 8006 Zurich, Switzerland)

  • Juerg Laederach

    (University of Zurich
    Present address: Institute of Molecular Biology and Biophysics, ETH Zurich, Otto Stern-Weg 5, 8093 Zurich, Switzerland)

  • Raimund Dutzler

    (University of Zurich)

Abstract

Secondary active transporters of the SLC11/NRAMP family catalyse the uptake of iron and manganese into cells. These proteins are highly conserved across all kingdoms of life and thus likely share a common transport mechanism. Here we describe the structural and functional properties of the prokaryotic SLC11 transporter EcoDMT. Its crystal structure reveals a previously unknown outward-facing state of the protein family. In proteoliposomes EcoDMT mediates proton-coupled uptake of manganese at low micromolar concentrations. Mutants of residues in the transition-metal ion-binding site severely affect transport, whereas a mutation of a conserved histidine located near this site results in metal ion transport that appears uncoupled to proton transport. Combined with previous results, our study defines the conformational changes underlying transition-metal ion transport in the SLC11 family and it provides molecular insight to its coupling to protons.

Suggested Citation

  • Ines A. Ehrnstorfer & Cristina Manatschal & Fabian M. Arnold & Juerg Laederach & Raimund Dutzler, 2017. "Structural and mechanistic basis of proton-coupled metal ion transport in the SLC11/NRAMP family," Nature Communications, Nature, vol. 8(1), pages 1-11, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14033
    DOI: 10.1038/ncomms14033
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    Cited by:

    1. Márton Liziczai & Ariane Fuchs & Cristina Manatschal & Raimund Dutzler, 2025. "Structural basis for metal ion transport by the human SLC11 proteins DMT1 and NRAMP1," Nature Communications, Nature, vol. 16(1), pages 1-16, December.

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