IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v8y2017i1d10.1038_ncomms14023.html
   My bibliography  Save this article

Identification of microsporidia host-exposed proteins reveals a repertoire of rapidly evolving proteins

Author

Listed:
  • Aaron W. Reinke

    (Section of Cell and Developmental Biology, University of California, San Diego)

  • Keir M. Balla

    (Section of Cell and Developmental Biology, University of California, San Diego)

  • Eric J. Bennett

    (Section of Cell and Developmental Biology, University of California, San Diego)

  • Emily R. Troemel

    (Section of Cell and Developmental Biology, University of California, San Diego)

Abstract

Pathogens use a variety of secreted and surface proteins to interact with and manipulate their hosts, but a systematic approach for identifying such proteins has been lacking. To identify these ‘host-exposed’ proteins, we used spatially restricted enzymatic tagging followed by mass spectrometry analysis of Caenorhabditis elegans infected with two species of Nematocida microsporidia. We identified 82 microsporidia proteins inside of intestinal cells, including several pathogen proteins in the nucleus. These microsporidia proteins are enriched in targeting signals, are rapidly evolving and belong to large Nematocida-specific gene families. We also find that large, species-specific families are common throughout microsporidia species. Our data suggest that the use of a large number of rapidly evolving species-specific proteins represents a common strategy for microsporidia to interact with their hosts. The unbiased method described here for identifying potential pathogen effectors represents a powerful approach to study a broad range of pathogens.

Suggested Citation

  • Aaron W. Reinke & Keir M. Balla & Eric J. Bennett & Emily R. Troemel, 2017. "Identification of microsporidia host-exposed proteins reveals a repertoire of rapidly evolving proteins," Nature Communications, Nature, vol. 8(1), pages 1-11, April.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14023
    DOI: 10.1038/ncomms14023
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms14023
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms14023?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Noelle V. Antao & Cherry Lam & Ari Davydov & Margot Riggi & Joseph Sall & Christopher Petzold & Feng-Xia Liang & Janet H. Iwasa & Damian C. Ekiert & Gira Bhabha, 2023. "3D reconstructions of parasite development and the intracellular niche of the microsporidian pathogen Encephalitozoon intestinalis," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms14023. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.