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Proximity does not contribute to activity enhancement in the glucose oxidase–horseradish peroxidase cascade

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  • Yifei Zhang

    (Columbia University)

  • Stanislav Tsitkov

    (Columbia University)

  • Henry Hess

    (Columbia University)

Abstract

A proximity effect has been invoked to explain the enhanced activity of enzyme cascades on DNA scaffolds. Using the cascade reaction carried out by glucose oxidase and horseradish peroxidase as a model system, here we study the kinetics of the cascade reaction when the enzymes are free in solution, when they are conjugated to each other and when a competing enzyme is present. No proximity effect is found, which is in agreement with models predicting that the rapidly diffusing hydrogen peroxide intermediate is well mixed. We suggest that the reason for the activity enhancement of enzymes localized by DNA scaffolds is that the pH near the surface of the negatively charged DNA nanostructures is lower than that in the bulk solution, creating a more optimal pH environment for the anchored enzymes. Our findings challenge the notion of a proximity effect and provide new insights into the role of DNA scaffolds.

Suggested Citation

  • Yifei Zhang & Stanislav Tsitkov & Henry Hess, 2016. "Proximity does not contribute to activity enhancement in the glucose oxidase–horseradish peroxidase cascade," Nature Communications, Nature, vol. 7(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13982
    DOI: 10.1038/ncomms13982
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    Cited by:

    1. Joyce C. Breger & James N. Vranish & Eunkeu Oh & Michael H. Stewart & Kimihiro Susumu & Guillermo Lasarte-Aragonés & Gregory A. Ellis & Scott A. Walper & Sebastián A. Díaz & Shelby L. Hooe & William , 2023. "Self assembling nanoparticle enzyme clusters provide access to substrate channeling in multienzymatic cascades," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    2. Prabhu Dhasaiyan & Tanwistha Ghosh & Hong-Guen Lee & Yeonsang Lee & Ilha Hwang & Rahul Dev Mukhopadhyay & Kyeng Min Park & Seungwon Shin & In Seok Kang & Kimoon Kim, 2022. "Cascade reaction networks within audible sound induced transient domains in a solution," Nature Communications, Nature, vol. 13(1), pages 1-10, December.

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