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A programmable DNA origami nanospring that reveals force-induced adjacent binding of myosin VI heads

Author

Listed:
  • M. Iwaki

    (Quantitative Biology Center, RIKEN
    Graduate School of Frontier Biosciences, Osaka University)

  • S. F. Wickham

    (Dana-Farber Cancer Institute
    Harvard Medical School
    Wyss Institute for Biologically Inspired Engineering, Harvard University)

  • K. Ikezaki

    (School of Frontier Sciences, The University of Tokyo)

  • T. Yanagida

    (Quantitative Biology Center, RIKEN
    Graduate School of Frontier Biosciences, Osaka University
    Center for Information and Neural Networks, NICT)

  • W. M. Shih

    (Dana-Farber Cancer Institute
    Harvard Medical School
    Wyss Institute for Biologically Inspired Engineering, Harvard University)

Abstract

Mechanosensitive biological nanomachines such as motor proteins and ion channels regulate diverse cellular behaviour. Combined optical trapping with single-molecule fluorescence imaging provides a powerful methodology to clearly characterize the mechanoresponse, structural dynamics and stability of such nanomachines. However, this system requires complicated experimental geometry, preparation and optics, and is limited by low data-acquisition efficiency. Here we develop a programmable DNA origami nanospring that overcomes these issues. We apply our nanospring to human myosin VI, a mechanosensory motor protein, and demonstrate nanometre-precision single-molecule fluorescence imaging of the individual motor domains (heads) under force. We observe force-induced transitions of myosin VI heads from non-adjacent to adjacent binding, which correspond to adapted roles for low-load and high-load transport, respectively. Our technique extends single-molecule studies under force and clarifies the effect of force on biological processes.

Suggested Citation

  • M. Iwaki & S. F. Wickham & K. Ikezaki & T. Yanagida & W. M. Shih, 2016. "A programmable DNA origami nanospring that reveals force-induced adjacent binding of myosin VI heads," Nature Communications, Nature, vol. 7(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13715
    DOI: 10.1038/ncomms13715
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    Cited by:

    1. Deepak Karna & Eriko Mano & Jiahao Ji & Ibuki Kawamata & Yuki Suzuki & Hanbin Mao, 2023. "Chemo-mechanical forces modulate the topology dynamics of mesoscale DNA assemblies," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

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