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Detecting stoichiometry of macromolecular complexes in live cells using FRET

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  • Manu Ben-Johny

    (Calcium Signals Laboratory, The Johns Hopkins University School of Medicine)

  • Daniel N. Yue

    (Calcium Signals Laboratory, The Johns Hopkins University School of Medicine)

  • David T. Yue

    (Calcium Signals Laboratory, The Johns Hopkins University School of Medicine)

Abstract

The stoichiometry of macromolecular interactions is fundamental to cellular signalling yet challenging to detect from living cells. Fluorescence resonance energy transfer (FRET) is a powerful phenomenon for characterizing close-range interactions whereby a donor fluorophore transfers energy to a closely juxtaposed acceptor. Recognizing that FRET measured from the acceptor’s perspective reports a related but distinct quantity versus the donor, we utilize the ratiometric comparison of the two to obtain the stoichiometry of a complex. Applying this principle to the long-standing controversy of calmodulin binding to ion channels, we find a surprising Ca2+-induced switch in calmodulin stoichiometry with Ca2+ channels—one calmodulin binds at basal cytosolic Ca2+ levels while two calmodulins interact following Ca2+ elevation. This feature is curiously absent for the related Na channels, also potently regulated by calmodulin. Overall, our assay adds to a burgeoning toolkit to pursue quantitative biochemistry of dynamic signalling complexes in living cells.

Suggested Citation

  • Manu Ben-Johny & Daniel N. Yue & David T. Yue, 2016. "Detecting stoichiometry of macromolecular complexes in live cells using FRET," Nature Communications, Nature, vol. 7(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13709
    DOI: 10.1038/ncomms13709
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