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Multivalent contacts of the Hsp70 Ssb contribute to its architecture on ribosomes and nascent chain interaction

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  • Marie A. Hanebuth

    (Molecular Microbiology, University of Konstanz
    Konstanz Research School Chemical Biology, University of Konstanz)

  • Roman Kityk

    (Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance)

  • Sandra J. Fries

    (Molecular Microbiology, University of Konstanz
    Konstanz Research School Chemical Biology, University of Konstanz)

  • Alok Jain

    (University of Konstanz)

  • Allison Kriel

    (Stanford University)

  • Veronique Albanese

    (Stanford University)

  • Tancred Frickey

    (Applied Bioinformatics, University of Konstanz)

  • Christine Peter

    (University of Konstanz)

  • Matthias P. Mayer

    (Center for Molecular Biology of Heidelberg University (ZMBH), DKFZ-ZMBH Alliance)

  • Judith Frydman

    (Stanford University)

  • Elke Deuerling

    (Molecular Microbiology, University of Konstanz)

Abstract

Hsp70 chaperones assist de novo folding of newly synthesized proteins in all cells. In yeast, the specialized Hsp70 Ssb directly binds to ribosomes. The structural basis and functional mode of recruitment of Ssb to ribosomes is not understood. Here, we present the molecular details underlying ribosome binding of Ssb in Saccharomyces cerevisiae. This interaction is multifaceted, involving the co-chaperone RAC and two specific regions within Ssb characterized by positive charges. The C-terminus of Ssb mediates the key contact and a second attachment point is provided by a KRR-motif in the substrate binding domain. Strikingly, ribosome binding of Ssb is not essential. Autonomous ribosome attachment becomes necessary if RAC is absent, suggesting a dual mode of Ssb recruitment to nascent chains. We propose, that the multilayered ribosomal interaction allows positioning of Ssb in an optimal orientation to the tunnel exit guaranteeing an efficient nascent polypeptide interaction.

Suggested Citation

  • Marie A. Hanebuth & Roman Kityk & Sandra J. Fries & Alok Jain & Allison Kriel & Veronique Albanese & Tancred Frickey & Christine Peter & Matthias P. Mayer & Judith Frydman & Elke Deuerling, 2016. "Multivalent contacts of the Hsp70 Ssb contribute to its architecture on ribosomes and nascent chain interaction," Nature Communications, Nature, vol. 7(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13695
    DOI: 10.1038/ncomms13695
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    Cited by:

    1. Yan Chen & Bin Tsai & Ningning Li & Ning Gao, 2022. "Structural remodeling of ribosome associated Hsp40-Hsp70 chaperones during co-translational folding," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

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