IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v7y2016i1d10.1038_ncomms13642.html
   My bibliography  Save this article

Production of individualized V gene databases reveals high levels of immunoglobulin genetic diversity

Author

Listed:
  • Martin M. Corcoran

    (Tumor and Cell Biology, Karolinska Institutet)

  • Ganesh E. Phad

    (Tumor and Cell Biology, Karolinska Institutet)

  • Néstor Vázquez Bernat

    (Tumor and Cell Biology, Karolinska Institutet)

  • Christiane Stahl-Hennig

    (Deutsches Primatenzentrum GmbH (DPZ), Leibniz-Institute for Primate Research)

  • Noriyuki Sumida

    (Tumor and Cell Biology, Karolinska Institutet)

  • Mats A.A. Persson

    (Stockholm University)

  • Marcel Martin

    (Science for Life Laboratory, Stockholm University)

  • Gunilla B. Karlsson Hedestam

    (Tumor and Cell Biology, Karolinska Institutet)

Abstract

Comprehensive knowledge of immunoglobulin genetics is required to advance our understanding of B cell biology. Validated immunoglobulin variable (V) gene databases are close to completion only for human and mouse. We present a novel computational approach, IgDiscover, that identifies germline V genes from expressed repertoires to a specificity of 100%. IgDiscover uses a cluster identification process to produce candidate sequences that, once filtered, results in individualized germline V gene databases. IgDiscover was tested in multiple species, validated by genomic cloning and cross library comparisons and produces comprehensive gene databases even where limited genomic sequence is available. IgDiscover analysis of the allelic content of the Indian and Chinese-origin rhesus macaques reveals high levels of immunoglobulin gene diversity in this species. Further, we describe a novel human IGHV3-21 allele and confirm significant gene differences between Balb/c and C57BL6 mouse strains, demonstrating the power of IgDiscover as a germline V gene discovery tool.

Suggested Citation

  • Martin M. Corcoran & Ganesh E. Phad & Néstor Vázquez Bernat & Christiane Stahl-Hennig & Noriyuki Sumida & Mats A.A. Persson & Marcel Martin & Gunilla B. Karlsson Hedestam, 2016. "Production of individualized V gene databases reveals high levels of immunoglobulin genetic diversity," Nature Communications, Nature, vol. 7(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13642
    DOI: 10.1038/ncomms13642
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms13642
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms13642?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Fredrika Hellgren & Alberto Cagigi & Rodrigo Arcoverde Cerveira & Sebastian Ols & Theresa Kern & Ang Lin & Bengt Eriksson & Michael G. Dodds & Edith Jasny & Kim Schwendt & Conrad Freuling & Thomas Mül, 2023. "Unmodified rabies mRNA vaccine elicits high cross-neutralizing antibody titers and diverse B cell memory responses," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    2. Mark Chernyshev & Mrunal Sakharkar & Ruth I. Connor & Haley L. Dugan & Daniel J. Sheward & C. G. Rappazzo & Aron Stålmarck & Mattias N. E. Forsell & Peter F. Wright & Martin Corcoran & Ben Murrell & L, 2023. "Vaccination of SARS-CoV-2-infected individuals expands a broad range of clonally diverse affinity-matured B cell lineages," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    3. Marco Mandolesi & Hrishikesh Das & Liset Vries & Yiqiu Yang & Changil Kim & Manojj Dhinakaran & Xaquin Castro Dopico & Julian Fischbach & Sungyong Kim & Mariia V. Guryleva & Monika Àdori & Mark Cherny, 2024. "Multi-compartmental diversification of neutralizing antibody lineages dissected in SARS-CoV-2 spike-immunized macaques," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13642. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.