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Unlocking sperm chromatin at fertilization requires a dedicated egg thioredoxin in Drosophila

Author

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  • Samantha Tirmarche

    (Laboratoire de Biométrie et Biologie Évolutive, Université de Lyon)

  • Shuhei Kimura

    (Laboratoire de Biométrie et Biologie Évolutive, Université de Lyon)

  • Raphaëlle Dubruille

    (Laboratoire de Biométrie et Biologie Évolutive, Université de Lyon)

  • Béatrice Horard

    (Laboratoire de Biométrie et Biologie Évolutive, Université de Lyon)

  • Benjamin Loppin

    (Laboratoire de Biométrie et Biologie Évolutive, Université de Lyon)

Abstract

In most animals, the extreme compaction of sperm DNA is achieved after the massive replacement of histones with sperm nuclear basic proteins (SNBPs), such as protamines. In some species, the ultracompact sperm chromatin is stabilized by a network of disulfide bonds connecting cysteine residues present in SNBPs. Studies in mammals have established that the reduction of these disulfide crosslinks at fertilization is required for sperm nuclear decondensation and the formation of the male pronucleus. Here, we show that the Drosophila maternal thioredoxin Deadhead (DHD) is specifically required to unlock sperm chromatin at fertilization. In dhd mutant eggs, the sperm nucleus fails to decondense and the replacement of SNBPs with maternally-provided histones is severely delayed, thus preventing the participation of paternal chromosomes in embryo development. We demonstrate that DHD localizes to the sperm nucleus to reduce its disulfide targets and is then rapidly degraded after fertilization.

Suggested Citation

  • Samantha Tirmarche & Shuhei Kimura & Raphaëlle Dubruille & Béatrice Horard & Benjamin Loppin, 2016. "Unlocking sperm chromatin at fertilization requires a dedicated egg thioredoxin in Drosophila," Nature Communications, Nature, vol. 7(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13539
    DOI: 10.1038/ncomms13539
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    Cited by:

    1. Guillermo A. Orsi & Maxime M. C. Tortora & Béatrice Horard & Dominique Baas & Jean-Philippe Kleman & Jonas Bucevičius & Gražvydas Lukinavičius & Daniel Jost & Benjamin Loppin, 2023. "Biophysical ordering transitions underlie genome 3D re-organization during cricket spermiogenesis," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

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