IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v7y2016i1d10.1038_ncomms13324.html
   My bibliography  Save this article

Saponin-based adjuvants induce cross-presentation in dendritic cells by intracellular lipid body formation

Author

Listed:
  • Martijn H. den Brok

    (Radboud Institute for Molecular Life Sciences, Radboud UMC, Geert Grooteplein 26, 6525 GA
    Pain and Palliative Medicine, Radboud UMC, Geert Grooteplein 10)

  • Christian Büll

    (Radboud Institute for Molecular Life Sciences, Radboud UMC, Geert Grooteplein 26, 6525 GA)

  • Melissa Wassink

    (Radboud Institute for Molecular Life Sciences, Radboud UMC, Geert Grooteplein 26, 6525 GA)

  • Annemarie M. de Graaf

    (Radboud Institute for Molecular Life Sciences, Radboud UMC, Geert Grooteplein 26, 6525 GA)

  • Jori A. Wagenaars

    (Radboud Institute for Molecular Life Sciences, Radboud UMC, Geert Grooteplein 26, 6525 GA)

  • Marthe Minderman

    (Radboud Institute for Molecular Life Sciences, Radboud UMC, Geert Grooteplein 26, 6525 GA)

  • Mayank Thakur

    (Institute for Laboratory Medicine, Clinical Chemistry and Pathobiochemistry, Charité Universitätsmedizin, Augustenburger Platz 1, 13353)

  • Sebastian Amigorena

    (INSERM, Institut Curie, Section Recherche, Rue d'Ulm 26, 75005)

  • Eric O. Rijke

    (MSD Animal Health)

  • Carla C. Schrier

    (MSD Animal Health)

  • Gosse J. Adema

    (Radboud Institute for Molecular Life Sciences, Radboud UMC, Geert Grooteplein 26, 6525 GA)

Abstract

Saponin-based adjuvants (SBAs) are being used in animal and human (cancer) vaccines, as they induce protective cellular immunity. Their adjuvant potency is a factor of inflammasome activation and enhanced antigen cross-presentation by dendritic cells (DCs), but how antigen cross-presentation is induced is not clear. Here we show that SBAs uniquely induce intracellular lipid bodies (LBs) in the CD11b+ DC subset in vitro and in vivo. Using genetic and pharmacological interference in models for vaccination and in situ tumour ablation, we demonstrate that LB induction is causally related to the saponin-dependent increase in cross-presentation and T-cell activation. These findings link adjuvant activity to LB formation, aid the application of SBAs as a cancer vaccine component, and will stimulate development of new adjuvants enhancing T-cell-mediated immunity.

Suggested Citation

  • Martijn H. den Brok & Christian Büll & Melissa Wassink & Annemarie M. de Graaf & Jori A. Wagenaars & Marthe Minderman & Mayank Thakur & Sebastian Amigorena & Eric O. Rijke & Carla C. Schrier & Gosse J, 2016. "Saponin-based adjuvants induce cross-presentation in dendritic cells by intracellular lipid body formation," Nature Communications, Nature, vol. 7(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13324
    DOI: 10.1038/ncomms13324
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms13324
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms13324?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Diego Calzada-Fraile & Salvador Iborra & Marta Ramírez-Huesca & Inmaculada Jorge & Enrico Dotta & Elena Hernández-García & Noa Martín-Cófreces & Estanislao Nistal-Villán & Esteban Veiga & Jesús Vázque, 2023. "Immune synapse formation promotes lipid peroxidation and MHC-I upregulation in licensed dendritic cells for efficient priming of CD8+ T cells," Nature Communications, Nature, vol. 14(1), pages 1-16, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13324. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.