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DciA is an ancestral replicative helicase operator essential for bacterial replication initiation

Author

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  • Pierre Brézellec

    (Universite de Versailles Saint-Quentin)

  • Isabelle Vallet-Gely

    (Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris‐Sud, Université Paris‐Saclay)

  • Christophe Possoz

    (Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris‐Sud, Université Paris‐Saclay)

  • Sophie Quevillon-Cheruel

    (Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris‐Sud, Université Paris‐Saclay)

  • Jean-Luc Ferat

    (Universite de Versailles Saint-Quentin
    Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris‐Sud, Université Paris‐Saclay)

Abstract

Delivery of the replicative helicase onto DNA is an essential step in the initiation of replication. In bacteria, DnaC (in Escherichia coli) and DnaI (in Bacillus subtilis) are representative of the two known mechanisms that assist the replicative helicase at this stage. Here, we establish that these two strategies cannot be regarded as prototypical of the bacterial domain since dnaC and dnaI (dna[CI]) are present in only a few bacterial phyla. We show that dna[CI] was domesticated at least seven times through evolution in bacteria and at the expense of one gene, which we rename dciA (dna[CI] antecedent), suggesting that DciA and Dna[CI] share a common function. We validate this hypothesis by establishing in Pseudomonas aeruginosa that DciA possesses the attributes of the replicative helicase-operating proteins associated with replication initiation.

Suggested Citation

  • Pierre Brézellec & Isabelle Vallet-Gely & Christophe Possoz & Sophie Quevillon-Cheruel & Jean-Luc Ferat, 2016. "DciA is an ancestral replicative helicase operator essential for bacterial replication initiation," Nature Communications, Nature, vol. 7(1), pages 1-7, December.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13271
    DOI: 10.1038/ncomms13271
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