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A direct-sensing galactose chemoreceptor recently evolved in invasive strains of Campylobacter jejuni

Author

Listed:
  • Christopher J. Day

    (Institute for Glycomics, Griffith University, Gold Coast Campus)

  • Rebecca M. King

    (Institute for Glycomics, Griffith University, Gold Coast Campus)

  • Lucy K. Shewell

    (Institute for Glycomics, Griffith University, Gold Coast Campus)

  • Greg Tram

    (Institute for Glycomics, Griffith University, Gold Coast Campus)

  • Tahria Najnin

    (Institute for Glycomics, Griffith University, Gold Coast Campus)

  • Lauren E. Hartley-Tassell

    (Institute for Glycomics, Griffith University, Gold Coast Campus)

  • Jennifer C. Wilson

    (School of Medical Science, Griffith University, Gold Coast Campus)

  • Aaron D. Fleetwood

    (University of Tennessee)

  • Igor B. Zhulin

    (University of Tennessee
    Oak Ridge National Laboratory)

  • Victoria Korolik

    (Institute for Glycomics, Griffith University, Gold Coast Campus
    School of Medical Science, Griffith University, Gold Coast Campus)

Abstract

A rare chemotaxis receptor, Tlp11, has been previously identified in invasive strains of Campylobacter jejuni, the most prevalent cause of bacterial gastroenteritis worldwide. Here we use glycan and small-molecule arrays, as well as surface plasmon resonance, to show that Tlp11 specifically interacts with galactose. Tlp11 is required for the chemotactic response of C. jejuni to galactose, as shown using wild type, allelic inactivation and addition mutants. The inactivated mutant displays reduced virulence in vivo, in a model of chicken colonization. The Tlp11 sensory domain represents the first known sugar-binding dCache_1 domain, which is the most abundant family of extracellular sensors in bacteria. The Tlp11 signalling domain interacts with the chemotaxis scaffolding proteins CheV and CheW, and comparative genomic analysis indicates a likely recent evolutionary origin for Tlp11. We propose to rename Tlp11 as CcrG, Campylobacter ChemoReceptor for Galactose.

Suggested Citation

  • Christopher J. Day & Rebecca M. King & Lucy K. Shewell & Greg Tram & Tahria Najnin & Lauren E. Hartley-Tassell & Jennifer C. Wilson & Aaron D. Fleetwood & Igor B. Zhulin & Victoria Korolik, 2016. "A direct-sensing galactose chemoreceptor recently evolved in invasive strains of Campylobacter jejuni," Nature Communications, Nature, vol. 7(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13206
    DOI: 10.1038/ncomms13206
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