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NIN-like protein 8 is a master regulator of nitrate-promoted seed germination in Arabidopsis

Author

Listed:
  • Dawei Yan

    (University of Toronto)

  • Vanathy Easwaran

    (University of Toronto)

  • Vivian Chau

    (University of Toronto)

  • Masanori Okamoto

    (Arid Land Research Center, Tottori University
    PRESTO, Japan Science and Technology Agency)

  • Matthew Ierullo

    (University of Toronto)

  • Mitsuhiro Kimura

    (University of Toronto
    Present address: Department of Life Science and Biotechnology, The Faculty of Life and Environmental Science, Shimane University 1060, Nishikawatsu, Matsue-shi, Shimane 690-8504, Japan)

  • Akira Endo

    (University of Toronto
    Present address: The National Institute of Agrobiological Sciences (NIAS), 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan)

  • Ryoichi Yano

    (Faculty of Life and Environmental Sciences, University of Tsukuba)

  • Asher Pasha

    (University of Toronto
    Centre for the Analysis of Genome Evolution and Function, University of Toronto)

  • Yunchen Gong

    (University of Toronto
    Centre for the Analysis of Genome Evolution and Function, University of Toronto)

  • Yong-Mei Bi

    (University of Guelph)

  • Nicolas Provart

    (University of Toronto
    Centre for the Analysis of Genome Evolution and Function, University of Toronto)

  • David Guttman

    (University of Toronto
    Centre for the Analysis of Genome Evolution and Function, University of Toronto)

  • Anne Krapp

    (Institut Jean-Pierre Bourgin, INRA, AgroParisTech, CNRS, Université Paris-Saclay)

  • Steven J. Rothstein

    (University of Guelph)

  • Eiji Nambara

    (University of Toronto
    Centre for the Analysis of Genome Evolution and Function, University of Toronto)

Abstract

Seeds respond to multiple different environmental stimuli that regulate germination. Nitrate stimulates germination in many plants but how it does so remains unclear. Here we show that the Arabidopsis NIN-like protein 8 (NLP8) is essential for nitrate-promoted seed germination. Seed germination in nlp8 loss-of-function mutants does not respond to nitrate. NLP8 functions even in a nitrate reductase-deficient mutant background, and the requirement for NLP8 is conserved among Arabidopsis accessions. NLP8 reduces abscisic acid levels in a nitrate-dependent manner and directly binds to the promoter of CYP707A2, encoding an abscisic acid catabolic enzyme. Genetic analysis shows that NLP8-mediated promotion of seed germination by nitrate requires CYP707A2. Finally, we show that NLP8 localizes to nuclei and unlike NLP7, does not appear to be activated by nitrate-dependent nuclear retention of NLP7, suggesting that seeds have a unique mechanism for nitrate signalling.

Suggested Citation

  • Dawei Yan & Vanathy Easwaran & Vivian Chau & Masanori Okamoto & Matthew Ierullo & Mitsuhiro Kimura & Akira Endo & Ryoichi Yano & Asher Pasha & Yunchen Gong & Yong-Mei Bi & Nicolas Provart & David Gutt, 2016. "NIN-like protein 8 is a master regulator of nitrate-promoted seed germination in Arabidopsis," Nature Communications, Nature, vol. 7(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13179
    DOI: 10.1038/ncomms13179
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