IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v7y2016i1d10.1038_ncomms13135.html
   My bibliography  Save this article

Nicotinamide is an endogenous agonist for a C. elegans TRPV OSM-9 and OCR-4 channel

Author

Listed:
  • Awani Upadhyay

    (The Pennsylvania State University
    Molecular Cellular and Integrative Biosciences Graduate Program, The Pennsylvania State University)

  • Aditya Pisupati

    (The Pennsylvania State University)

  • Timothy Jegla

    (The Pennsylvania State University)

  • Matt Crook

    (The Pennsylvania State University
    Present address: Department of Biology, Whitman College, Walla Walla, Washington 99362, USA)

  • Keith J. Mickolajczyk

    (The Pennsylvania State University
    Interdisciplinary Graduate Degree Program in Bioengineering, The Pennsylvania State University)

  • Matthew Shorey

    (The Pennsylvania State University
    Molecular Cellular and Integrative Biosciences Graduate Program, The Pennsylvania State University)

  • Laura E. Rohan

    (The Pennsylvania State University)

  • Katherine A. Billings

    (The Pennsylvania State University)

  • Melissa M. Rolls

    (The Pennsylvania State University)

  • William O. Hancock

    (The Pennsylvania State University)

  • Wendy Hanna-Rose

    (The Pennsylvania State University)

Abstract

TRPV ion channels are directly activated by sensory stimuli and participate in thermo-, mechano- and chemo-sensation. They are also hypothesized to respond to endogenous agonists that would modulate sensory responses. Here, we show that the nicotinamide (NAM) form of vitamin B3 is an agonist of a Caenorhabditis elegans TRPV channel. Using heterologous expression in Xenopus oocytes, we demonstrate that NAM is a soluble agonist for a channel consisting of the well-studied OSM-9 TRPV subunit and relatively uncharacterized OCR-4 TRPV subunit as well as the orthologous Drosophila Nan-Iav TRPV channel, and we examine stoichiometry of subunit assembly. Finally, we show that behaviours mediated by these C. elegans and Drosophila channels are responsive to NAM, suggesting conservation of activity of this soluble endogenous metabolite on TRPV activity. Our results in combination with the role of NAM in NAD+ metabolism suggest an intriguing link between metabolic regulation and TRPV channel activity.

Suggested Citation

  • Awani Upadhyay & Aditya Pisupati & Timothy Jegla & Matt Crook & Keith J. Mickolajczyk & Matthew Shorey & Laura E. Rohan & Katherine A. Billings & Melissa M. Rolls & William O. Hancock & Wendy Hanna-Ro, 2016. "Nicotinamide is an endogenous agonist for a C. elegans TRPV OSM-9 and OCR-4 channel," Nature Communications, Nature, vol. 7(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13135
    DOI: 10.1038/ncomms13135
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms13135
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms13135?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Martina Nicoletti & Letizia Chiodo & Alessandro Loppini, 2021. "Biophysics and Modeling of Mechanotransduction in Neurons: A Review," Mathematics, MDPI, vol. 9(4), pages 1-32, February.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms13135. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.