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H2A.Z controls the stability and mobility of nucleosomes to regulate expression of the LH genes

Author

Listed:
  • Sergei Rudnizky

    (Faculty of Biology, Technion—Israel Institute of Technology)

  • Adaiah Bavly

    (Faculty of Biology, Technion—Israel Institute of Technology)

  • Omri Malik

    (Russell Berrie Nanotechnology Institute, Technion—Israel Institute of Technology)

  • Lilach Pnueli

    (Faculty of Biology, Technion—Israel Institute of Technology)

  • Philippa Melamed

    (Faculty of Biology, Technion—Israel Institute of Technology
    Russell Berrie Nanotechnology Institute, Technion—Israel Institute of Technology)

  • Ariel Kaplan

    (Faculty of Biology, Technion—Israel Institute of Technology
    Russell Berrie Nanotechnology Institute, Technion—Israel Institute of Technology)

Abstract

The structure and dynamics of promoter chromatin have a profound effect on the expression levels of genes. Yet, the contribution of DNA sequence, histone post-translational modifications, histone variant usage and other factors in shaping the architecture of chromatin, and the mechanisms by which this architecture modulates expression of specific genes are not yet completely understood. Here we use optical tweezers to study the roles that DNA sequence and the histone variant H2A.Z have in shaping the chromatin landscape at the promoters of two model genes, Cga and Lhb. Guided by MNase mapping of the promoters of these genes, we reconstitute nucleosomes that mimic those located near the transcriptional start site and immediately downstream (+1), and measure the forces required to disrupt these nucleosomes, and their mobility along the DNA sequence. Our results indicate that these genes are basally regulated by two distinct strategies, making use of H2A.Z to modulate separate phases of transcription, and highlight how DNA sequence, alternative histone variants and remodelling machinery act synergistically to modulate gene expression.

Suggested Citation

  • Sergei Rudnizky & Adaiah Bavly & Omri Malik & Lilach Pnueli & Philippa Melamed & Ariel Kaplan, 2016. "H2A.Z controls the stability and mobility of nucleosomes to regulate expression of the LH genes," Nature Communications, Nature, vol. 7(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12958
    DOI: 10.1038/ncomms12958
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    Cited by:

    1. László Imre & Péter Nánási & Ibtissem Benhamza & Kata Nóra Enyedi & Gábor Mocsár & Rosevalentine Bosire & Éva Hegedüs & Erfaneh Firouzi Niaki & Ágota Csóti & Zsuzsanna Darula & Éva Csősz & Szilárd Pól, 2024. "Epigenetic modulation via the C-terminal tail of H2A.Z," Nature Communications, Nature, vol. 15(1), pages 1-21, December.
    2. Rani Zananiri & Sivasubramanyan Mangapuram Venkata & Vera Gaydar & Dan Yahalom & Omri Malik & Sergei Rudnizky & Oded Kleifeld & Ariel Kaplan & Arnon Henn, 2022. "Auxiliary ATP binding sites support DNA unwinding by RecBCD," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    3. Shuxiang Li & Tiejun Wei & Anna R. Panchenko, 2023. "Histone variant H2A.Z modulates nucleosome dynamics to promote DNA accessibility," Nature Communications, Nature, vol. 14(1), pages 1-10, December.

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