IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v7y2016i1d10.1038_ncomms12810.html
   My bibliography  Save this article

Cortical dynamics during cell motility are regulated by CRL3KLHL21 E3 ubiquitin ligase

Author

Listed:
  • Thibault Courtheoux

    (Institute of Biochemistry, ETH Zurich)

  • Radoslav I. Enchev

    (Institute of Biochemistry, ETH Zurich)

  • Fabienne Lampert

    (Institute of Biochemistry, ETH Zurich)

  • Juan Gerez

    (Institute of Biochemistry, ETH Zurich)

  • Jochen Beck

    (Institute of Biochemistry, ETH Zurich)

  • Paola Picotti

    (Institute of Biochemistry, ETH Zurich)

  • Izabela Sumara

    (Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Centre National de la Recherche Scientifique UMR 7104, Institut National de la Santé et de la Recherche Médicale U964, Université de Strasbourg)

  • Matthias Peter

    (Institute of Biochemistry, ETH Zurich)

Abstract

Directed cell movement involves spatial and temporal regulation of the cortical microtubule (Mt) and actin networks to allow focal adhesions (FAs) to assemble at the cell front and disassemble at the rear. Mts are known to associate with FAs, but the mechanisms coordinating their dynamic interactions remain unknown. Here we show that the CRL3KLHL21 E3 ubiquitin ligase promotes cell migration by controlling Mt and FA dynamics at the cell cortex. Indeed, KLHL21 localizes to FA structures preferentially at the leading edge, and in complex with Cul3, ubiquitylates EB1 within its microtubule-interacting CH-domain. Cells lacking CRL3KLHL21 activity or expressing a non-ubiquitylatable EB1 mutant protein are unable to migrate and exhibit strong defects in FA dynamics, lamellipodia formation and cortical plasticity. Our study thus reveals an important mechanism to regulate cortical dynamics during cell migration that involves ubiquitylation of EB1 at focal adhesions.

Suggested Citation

  • Thibault Courtheoux & Radoslav I. Enchev & Fabienne Lampert & Juan Gerez & Jochen Beck & Paola Picotti & Izabela Sumara & Matthias Peter, 2016. "Cortical dynamics during cell motility are regulated by CRL3KLHL21 E3 ubiquitin ligase," Nature Communications, Nature, vol. 7(1), pages 1-13, November.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12810
    DOI: 10.1038/ncomms12810
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms12810
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms12810?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12810. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.