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Crystal structure and functional characterization of a light-driven chloride pump having an NTQ motif

Author

Listed:
  • Kuglae Kim

    (Yonsei University)

  • Soon-Kyeong Kwon

    (Yonsei University)

  • Sung-Hoon Jun

    (Yonsei University)

  • Jeong Seok Cha

    (Yonsei University)

  • Hoyoung Kim

    (Yonsei University)

  • Weontae Lee

    (Yonsei University)

  • Jihyun F. Kim

    (Yonsei University
    Strategic Initiative for Microbiomes in Agriculture and Food, Yonsei University)

  • Hyun-Soo Cho

    (Yonsei University)

Abstract

A novel light-driven chloride-pumping rhodopsin (ClR) containing an ‘NTQ motif’ in its putative ion conduction pathway has been discovered and functionally characterized in a genomic analysis study of a marine bacterium. Here we report the crystal structure of ClR from the flavobacterium Nonlabens marinus S1-08T determined under two conditions at 2.0 and 1.56 Å resolutions. The structures reveal two chloride-binding sites, one around the protonated Schiff base and the other on a cytoplasmic loop. We identify a ‘3 omega motif’ formed by three non-consecutive aromatic amino acids that is correlated with the B–C loop orientation. Detailed ClR structural analyses with functional studies in E. coli reveal the chloride ion transduction pathway. Our results help understand the molecular mechanism and physiological role of ClR and provide a structural basis for optogenetic applications.

Suggested Citation

  • Kuglae Kim & Soon-Kyeong Kwon & Sung-Hoon Jun & Jeong Seok Cha & Hoyoung Kim & Weontae Lee & Jihyun F. Kim & Hyun-Soo Cho, 2016. "Crystal structure and functional characterization of a light-driven chloride pump having an NTQ motif," Nature Communications, Nature, vol. 7(1), pages 1-10, November.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12677
    DOI: 10.1038/ncomms12677
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    Cited by:

    1. R. Astashkin & K. Kovalev & S. Bukhdruker & S. Vaganova & A. Kuzmin & A. Alekseev & T. Balandin & D. Zabelskii & I. Gushchin & A. Royant & D. Volkov & G. Bourenkov & E. Koonin & M. Engelhard & E. Bamb, 2022. "Structural insights into light-driven anion pumping in cyanobacteria," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

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