IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v7y2016i1d10.1038_ncomms12648.html
   My bibliography  Save this article

Ubiquitin ligase RNF20/40 facilitates spindle assembly and promotes breast carcinogenesis through stabilizing motor protein Eg5

Author

Listed:
  • Yang Duan

    (Tianjin Key Laboratory of Medical Epigenetics, Tianjin Medical University)

  • Dawei Huo

    (Tianjin Key Laboratory of Medical Epigenetics, Tianjin Medical University)

  • Jie Gao

    (Tianjin Key Laboratory of Medical Epigenetics, Tianjin Medical University)

  • Heng Wu

    (Tianjin Key Laboratory of Lung Cancer Metastasis and Tumour Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital)

  • Zheng Ye

    (Tianjin Key Laboratory of Medical Epigenetics, Tianjin Medical University)

  • Zhe Liu

    (Tianjin Medical University)

  • Kai Zhang

    (Tianjin Key Laboratory of Medical Epigenetics, Tianjin Medical University)

  • Lin Shan

    (Tianjin Key Laboratory of Medical Epigenetics, Tianjin Medical University)

  • Xing Zhou

    (Tianjin Key Laboratory of Medical Epigenetics, Tianjin Medical University)

  • Yue Wang

    (Tianjin Key Laboratory of Medical Epigenetics, Tianjin Medical University)

  • Dongxue Su

    (Tianjin Key Laboratory of Medical Epigenetics, Tianjin Medical University)

  • Xiang Ding

    (Institute of Biophysics, Chinese Academy of Sciences)

  • Lei Shi

    (Tianjin Key Laboratory of Medical Epigenetics, Tianjin Medical University)

  • Yan Wang

    (Tianjin Key Laboratory of Medical Epigenetics, Tianjin Medical University)

  • Yongfeng Shang

    (Tianjin Key Laboratory of Medical Epigenetics, Tianjin Medical University
    Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Health Science Center)

  • Chenghao Xuan

    (Tianjin Key Laboratory of Medical Epigenetics, Tianjin Medical University)

Abstract

Whether transcriptional regulators are functionally involved in mitosis is a fundamental question in cell biology. Here we report that the RNF20/40 complex, a major ubiquitin ligase catalysing histone H2B monoubiquitination, interacts with the motor protein Eg5 during mitosis and participates in spindle assembly. We show that the RNF20/40 complex monoubiquitinates and stabilizes Eg5. Loss of RNF20/40 results in spindle assembly defects, cell cycle arrest and apoptosis. Consistently, depletion of either RNF20/40 or Eg5 suppresses breast cancer in vivo. Significantly, RNF20/40 and Eg5 are concurrently upregulated in human breast carcinomas and high Eg5 expression is associated with poorer overall survival of patients with luminal A, or B, breast cancer. Our study uncovers an important spindle assembly role of the RNF20/40 complex, and implicates the RNF20/40-Eg5 axis in breast carcinogenesis, supporting the pursuit of these proteins as potential targets for breast cancer therapeutic interventions.

Suggested Citation

  • Yang Duan & Dawei Huo & Jie Gao & Heng Wu & Zheng Ye & Zhe Liu & Kai Zhang & Lin Shan & Xing Zhou & Yue Wang & Dongxue Su & Xiang Ding & Lei Shi & Yan Wang & Yongfeng Shang & Chenghao Xuan, 2016. "Ubiquitin ligase RNF20/40 facilitates spindle assembly and promotes breast carcinogenesis through stabilizing motor protein Eg5," Nature Communications, Nature, vol. 7(1), pages 1-14, November.
  • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12648
    DOI: 10.1038/ncomms12648
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms12648
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/ncomms12648?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Yong Geun Jeon & Hahn Nahmgoong & Jiyoung Oh & Dabin Lee & Dong Wook Kim & Jane Eunsoo Kim & Ye Young Kim & Yul Ji & Ji Seul Han & Sung Min Kim & Jee Hyung Sohn & Won Taek Lee & Sun Won Kim & Jeu Park, 2024. "Ubiquitin ligase RNF20 coordinates sequential adipose thermogenesis with brown and beige fat-specific substrates," Nature Communications, Nature, vol. 15(1), pages 1-17, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12648. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.