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Integrative proteomic profiling of ovarian cancer cell lines reveals precursor cell associated proteins and functional status

Author

Listed:
  • F. Coscia

    (Max Planck Institute of Biochemistry)

  • K. M. Watters

    (Section of Gynecologic Oncology, University of Chicago)

  • M. Curtis

    (Section of Gynecologic Oncology, University of Chicago)

  • M. A. Eckert

    (Section of Gynecologic Oncology, University of Chicago)

  • C. Y. Chiang

    (Section of Gynecologic Oncology, University of Chicago)

  • S. Tyanova

    (Max Planck Institute of Biochemistry)

  • A. Montag

    (University of Chicago Medicine)

  • R. R. Lastra

    (University of Chicago Medicine)

  • E. Lengyel

    (Section of Gynecologic Oncology, University of Chicago)

  • M. Mann

    (Max Planck Institute of Biochemistry)

Abstract

A cell line representative of human high-grade serous ovarian cancer (HGSOC) should not only resemble its tumour of origin at the molecular level, but also demonstrate functional utility in pre-clinical investigations. Here, we report the integrated proteomic analysis of 26 ovarian cancer cell lines, HGSOC tumours, immortalized ovarian surface epithelial cells and fallopian tube epithelial cells via a single-run mass spectrometric workflow. The in-depth quantification of >10,000 proteins results in three distinct cell line categories: epithelial (group I), clear cell (group II) and mesenchymal (group III). We identify a 67-protein cell line signature, which separates our entire proteomic data set, as well as a confirmatory publicly available CPTAC/TCGA tumour proteome data set, into a predominantly epithelial and mesenchymal HGSOC tumour cluster. This proteomics-based epithelial/mesenchymal stratification of cell lines and human tumours indicates a possible origin of HGSOC either from the fallopian tube or from the ovarian surface epithelium.

Suggested Citation

  • F. Coscia & K. M. Watters & M. Curtis & M. A. Eckert & C. Y. Chiang & S. Tyanova & A. Montag & R. R. Lastra & E. Lengyel & M. Mann, 2016. "Integrative proteomic profiling of ovarian cancer cell lines reveals precursor cell associated proteins and functional status," Nature Communications, Nature, vol. 7(1), pages 1-14, November.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12645
    DOI: 10.1038/ncomms12645
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    Cited by:

    1. Nhung H Vuong & David P Cook & Laura A Forrest & Lauren E Carter & Pascale Robineau-Charette & Joshua M Kofsky & Kendra M Hodgkinson & Barbara C Vanderhyden, 2018. "Single-cell RNA-sequencing reveals transcriptional dynamics of estrogen-induced dysplasia in the ovarian surface epithelium," PLOS Genetics, Public Library of Science, vol. 14(11), pages 1-22, November.

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