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Long-term time-lapse microscopy of C. elegans post-embryonic development

Author

Listed:
  • Nicola Gritti

    (FOM Institute AMOLF)

  • Simone Kienle

    (FOM Institute AMOLF)

  • Olga Filina

    (FOM Institute AMOLF)

  • Jeroen Sebastiaan van Zon

    (FOM Institute AMOLF)

Abstract

We present a microscopy technique that enables long-term time-lapse microscopy at single-cell resolution in moving and feeding Caenorhabditis elegans larvae. Time-lapse microscopy of C. elegans post-embryonic development is challenging, as larvae are highly motile. Moreover, immobilization generally leads to rapid developmental arrest. Instead, we confine larval movement to microchambers that contain bacteria as food, and use fast image acquisition and image analysis to follow the dynamics of cells inside individual larvae, as they move within each microchamber. This allows us to perform fluorescence microscopy of 10–20 animals in parallel with 20 min time resolution. We demonstrate the power of our approach by analysing the dynamics of cell division, cell migration and gene expression over the full ∼48 h of development from larva to adult. Our approach now makes it possible to study the behaviour of individual cells inside the body of a feeding and growing animal.

Suggested Citation

  • Nicola Gritti & Simone Kienle & Olga Filina & Jeroen Sebastiaan van Zon, 2016. "Long-term time-lapse microscopy of C. elegans post-embryonic development," Nature Communications, Nature, vol. 7(1), pages 1-9, November.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12500
    DOI: 10.1038/ncomms12500
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    Cited by:

    1. Klement Stojanovski & Ioana Gheorghe & Peter Lenart & Anne Lanjuin & William B. Mair & Benjamin D. Towbin, 2023. "Maintenance of appropriate size scaling of the C. elegans pharynx by YAP-1," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    2. Damien G Hicks & Terence P Speed & Mohammed Yassin & Sarah M Russell, 2019. "Maps of variability in cell lineage trees," PLOS Computational Biology, Public Library of Science, vol. 15(2), pages 1-32, February.
    3. Klement Stojanovski & Helge Großhans & Benjamin D. Towbin, 2022. "Coupling of growth rate and developmental tempo reduces body size heterogeneity in C. elegans," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

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