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In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum

Author

Listed:
  • Jakob Toudahl Nielsen

    (Center for Insoluble Protein Structures (inSPIN)
    Interdisciplinary Nanoscience Center (iNANO), Aarhus University
    Department of Chemistry)

  • Natalia V. Kulminskaya

    (Center for Insoluble Protein Structures (inSPIN)
    Interdisciplinary Nanoscience Center (iNANO), Aarhus University
    Department of Chemistry)

  • Morten Bjerring

    (Center for Insoluble Protein Structures (inSPIN)
    Interdisciplinary Nanoscience Center (iNANO), Aarhus University
    Department of Chemistry)

  • Juha M. Linnanto

    (Institute of Physics, University of Tartu)

  • Margus Rätsep

    (Institute of Physics, University of Tartu)

  • Marie Østergaard Pedersen

    (Center for Insoluble Protein Structures (inSPIN)
    Novo Nordisk a/s, Novo Nordisk Park)

  • Petar H. Lambrev

    (Hungarian Academy of Sciences, Biological Research Centre)

  • Márta Dorogi

    (Hungarian Academy of Sciences, Biological Research Centre)

  • Győző Garab

    (Hungarian Academy of Sciences, Biological Research Centre)

  • Karen Thomsen

    (Interdisciplinary Nanoscience Center (iNANO), Aarhus University)

  • Caroline Jegerschöld

    (Karolinska Institutet)

  • Niels-Ulrik Frigaard

    (Section for Marine Biology, University of Copenhagen)

  • Martin Lindahl

    (Karolinska Institutet)

  • Niels Chr. Nielsen

    (Center for Insoluble Protein Structures (inSPIN)
    Interdisciplinary Nanoscience Center (iNANO), Aarhus University
    Department of Chemistry)

Abstract

Photosynthetic antenna systems enable organisms harvesting light and transfer the energy to the photosynthetic reaction centre, where the conversion to chemical energy takes place. One of the most complex antenna systems, the chlorosome, found in the photosynthetic green sulfur bacterium Chlorobaculum (Cba.) tepidum contains a baseplate, which is a scaffolding super-structure, formed by the protein CsmA and bacteriochlorophyll a. Here we present the first high-resolution structure of the CsmA baseplate using intact fully functional, light-harvesting organelles from Cba. tepidum, following a hybrid approach combining five complementary methods: solid-state NMR spectroscopy, cryo-electron microscopy, isotropic and anisotropic circular dichroism and linear dichroism. The structure calculation was facilitated through development of new software, GASyCS for efficient geometry optimization of highly symmetric oligomeric structures. We show that the baseplate is composed of rods of repeated dimers of the strongly amphipathic CsmA with pigments sandwiched within the dimer at the hydrophobic side of the helix.

Suggested Citation

  • Jakob Toudahl Nielsen & Natalia V. Kulminskaya & Morten Bjerring & Juha M. Linnanto & Margus Rätsep & Marie Østergaard Pedersen & Petar H. Lambrev & Márta Dorogi & Győző Garab & Karen Thomsen & Caroli, 2016. "In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum," Nature Communications, Nature, vol. 7(1), pages 1-12, November.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12454
    DOI: 10.1038/ncomms12454
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