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Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography

Author

Listed:
  • Przemyslaw Nogly

    (Laboratory for Biomolecular Research, Paul Scherrer Institute)

  • Valerie Panneels

    (Laboratory for Biomolecular Research, Paul Scherrer Institute)

  • Garrett Nelson

    (Arizona State University)

  • Cornelius Gati

    (Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY)

  • Tetsunari Kimura

    (Biometal Science Laboratory, RIKEN SPring-8 Center)

  • Christopher Milne

    (SwissFEL, Paul Scherrer Institute)

  • Despina Milathianaki

    (Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory)

  • Minoru Kubo

    (Biometal Science Laboratory, RIKEN SPring-8 Center
    PRESTO, JST)

  • Wenting Wu

    (Laboratory for Biomolecular Research, Paul Scherrer Institute)

  • Chelsie Conrad

    (and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University)

  • Jesse Coe

    (and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University)

  • Richard Bean

    (Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY)

  • Yun Zhao

    (Arizona State University)

  • Petra Båth

    (University of Gothenburg)

  • Robert Dods

    (University of Gothenburg)

  • Rajiv Harimoorthy

    (University of Gothenburg)

  • Kenneth R. Beyerlein

    (Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY)

  • Jan Rheinberger

    (Laboratory for Biomolecular Research, Paul Scherrer Institute)

  • Daniel James

    (Laboratory for Biomolecular Research, Paul Scherrer Institute)

  • Daniel DePonte

    (Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory)

  • Chufeng Li

    (Arizona State University)

  • Leonardo Sala

    (SwissFEL, Paul Scherrer Institute)

  • Garth J. Williams

    (Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory)

  • Mark S. Hunter

    (Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory)

  • Jason E. Koglin

    (Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory)

  • Peter Berntsen

    (University of Gothenburg)

  • Eriko Nango

    (SACLA Science Research Group, RIKEN/SPring-8 Center)

  • So Iwata

    (SACLA Science Research Group, RIKEN/SPring-8 Center
    Kyoto University)

  • Henry N. Chapman

    (Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY
    University of Hamburg
    Centre for Ultrafast Imaging, University of Hamburg)

  • Petra Fromme

    (and Center for Applied Structural Discovery, Biodesign Institute, Arizona State University)

  • Matthias Frank

    (Lawrence Livermore National Laboratory)

  • Rafael Abela

    (SwissFEL, Paul Scherrer Institute)

  • Sébastien Boutet

    (Linac Coherent Light Source (LCLS), SLAC National Accelerator Laboratory)

  • Anton Barty

    (Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY)

  • Thomas A. White

    (Center for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY)

  • Uwe Weierstall

    (Arizona State University)

  • John Spence

    (Arizona State University)

  • Richard Neutze

    (University of Gothenburg)

  • Gebhard Schertler

    (Laboratory for Biomolecular Research, Paul Scherrer Institute
    ETH Zurich)

  • Jörg Standfuss

    (Laboratory for Biomolecular Research, Paul Scherrer Institute)

Abstract

Serial femtosecond crystallography (SFX) using X-ray free-electron laser sources is an emerging method with considerable potential for time-resolved pump-probe experiments. Here we present a lipidic cubic phase SFX structure of the light-driven proton pump bacteriorhodopsin (bR) to 2.3 Å resolution and a method to investigate protein dynamics with modest sample requirement. Time-resolved SFX (TR-SFX) with a pump-probe delay of 1 ms yields difference Fourier maps compatible with the dark to M state transition of bR. Importantly, the method is very sample efficient and reduces sample consumption to about 1 mg per collected time point. Accumulation of M intermediate within the crystal lattice is confirmed by time-resolved visible absorption spectroscopy. This study provides an important step towards characterizing the complete photocycle dynamics of retinal proteins and demonstrates the feasibility of a sample efficient viscous medium jet for TR-SFX.

Suggested Citation

  • Przemyslaw Nogly & Valerie Panneels & Garrett Nelson & Cornelius Gati & Tetsunari Kimura & Christopher Milne & Despina Milathianaki & Minoru Kubo & Wenting Wu & Chelsie Conrad & Jesse Coe & Richard Be, 2016. "Lipidic cubic phase injector is a viable crystal delivery system for time-resolved serial crystallography," Nature Communications, Nature, vol. 7(1), pages 1-9, November.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12314
    DOI: 10.1038/ncomms12314
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    Cited by:

    1. Maximilian Wranik & Michal W. Kepa & Emma V. Beale & Daniel James & Quentin Bertrand & Tobias Weinert & Antonia Furrer & Hannah Glover & Dardan Gashi & Melissa Carrillo & Yasushi Kondo & Robin T. Stip, 2023. "A multi-reservoir extruder for time-resolved serial protein crystallography and compound screening at X-ray free-electron lasers," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

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