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Peptide–oligonucleotide conjugates as nanoscale building blocks for assembly of an artificial three-helix protein mimic

Author

Listed:
  • Chenguang Lou

    (Chemistry and Pharmacy, Biomolecular Nanoscale Engineering Center, University of Southern Denmark)

  • Manuel C. Martos-Maldonado

    (Biomolecular Nanoscale Engineering Center, University of Copenhagen)

  • Charlotte S. Madsen

    (Biomolecular Nanoscale Engineering Center, University of Copenhagen)

  • Rasmus P. Thomsen

    (Biomolecular Nanoscale Engineering Center and Interdisciplinary Nanoscience Center (iNANO), University of Aarhus)

  • Søren Roi Midtgaard

    (Niels Bohr Institute, University of Copenhagen)

  • Niels Johan Christensen

    (Biomolecular Nanoscale Engineering Center, University of Copenhagen)

  • Jørgen Kjems

    (Biomolecular Nanoscale Engineering Center and Interdisciplinary Nanoscience Center (iNANO), University of Aarhus)

  • Peter W. Thulstrup

    (University of Copenhagen)

  • Jesper Wengel

    (Chemistry and Pharmacy, Biomolecular Nanoscale Engineering Center, University of Southern Denmark)

  • Knud J. Jensen

    (Biomolecular Nanoscale Engineering Center, University of Copenhagen)

Abstract

Peptide-based structures can be designed to yield artificial proteins with specific folding patterns and functions. Template-based assembly of peptide units is one design option, but the use of two orthogonal self-assembly principles, oligonucleotide triple helix and a coiled coil protein domain formation have never been realized for de novo protein design. Here, we show the applicability of peptide–oligonucleotide conjugates for self-assembly of higher-ordered protein-like structures. The resulting nano-assemblies were characterized by ultraviolet-melting, gel electrophoresis, circular dichroism (CD) spectroscopy, small-angle X-ray scattering and transmission electron microscopy. These studies revealed the formation of the desired triple helix and coiled coil domains at low concentrations, while a dimer of trimers was dominating at high concentration. CD spectroscopy showed an extraordinarily high degree of α-helicity for the peptide moieties in the assemblies. The results validate the use of orthogonal self-assembly principles as a paradigm for de novo protein design.

Suggested Citation

  • Chenguang Lou & Manuel C. Martos-Maldonado & Charlotte S. Madsen & Rasmus P. Thomsen & Søren Roi Midtgaard & Niels Johan Christensen & Jørgen Kjems & Peter W. Thulstrup & Jesper Wengel & Knud J. Jense, 2016. "Peptide–oligonucleotide conjugates as nanoscale building blocks for assembly of an artificial three-helix protein mimic," Nature Communications, Nature, vol. 7(1), pages 1-9, November.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12294
    DOI: 10.1038/ncomms12294
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