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Load-induced enhancement of Dynein force production by LIS1–NudE in vivo and in vitro

Author

Listed:
  • Babu J. N. Reddy

    (University of California Irvine)

  • Michelle Mattson

    (University of California Irvine)

  • Caitlin L. Wynne

    (Columbia University)

  • Omid Vadpey

    (University of California Irvine)

  • Abdo Durra

    (University of California Irvine)

  • Dail Chapman

    (University of California Irvine)

  • Richard B. Vallee

    (Columbia University)

  • Steven P. Gross

    (University of California Irvine)

Abstract

Most sub-cellular cargos are transported along microtubules by kinesin and dynein molecular motors, but how transport is regulated is not well understood. It is unknown whether local control is possible, for example, by changes in specific cargo-associated motor behaviour to react to impediments. Here we discover that microtubule-associated lipid droplets (LDs) in COS1 cells respond to an optical trap with a remarkable enhancement in sustained force production. This effect is observed only for microtubule minus-end-moving LDs. It is specifically blocked by RNAi for the cytoplasmic dynein regulators LIS1 and NudE/L (Nde1/Ndel1), but not for the dynactin p150Glued subunit. It can be completely replicated using cell-free preparations of purified LDs, where duration of LD force production is more than doubled. These results identify a novel, intrinsic, cargo-associated mechanism for dynein-mediated force adaptation, which should markedly improve the ability of motor-driven cargoes to overcome subcellular obstacles.

Suggested Citation

  • Babu J. N. Reddy & Michelle Mattson & Caitlin L. Wynne & Omid Vadpey & Abdo Durra & Dail Chapman & Richard B. Vallee & Steven P. Gross, 2016. "Load-induced enhancement of Dynein force production by LIS1–NudE in vivo and in vitro," Nature Communications, Nature, vol. 7(1), pages 1-14, November.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12259
    DOI: 10.1038/ncomms12259
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