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Bacterial partition complexes segregate within the volume of the nucleoid

Author

Listed:
  • Antoine Le Gall

    (Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier)

  • Diego I. Cattoni

    (Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier)

  • Baptiste Guilhas

    (Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier)

  • Céline Mathieu-Demazière

    (Laboratoire de Microbiologie et Génétique Moléculaires, Centre de Biologie Intégrative, Centre National de la Recherche Scientifique, Université de Toulouse, UPS)

  • Laura Oudjedi

    (Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier)

  • Jean-Bernard Fiche

    (Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier)

  • Jérôme Rech

    (Laboratoire de Microbiologie et Génétique Moléculaires, Centre de Biologie Intégrative, Centre National de la Recherche Scientifique, Université de Toulouse, UPS)

  • Sara Abrahamsson

    (HHMI, Lulu and Anthony Wang Laboratory of Neural Circuits and Behavior, The Rockefeller University)

  • Heath Murray

    (Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University)

  • Jean-Yves Bouet

    (Laboratoire de Microbiologie et Génétique Moléculaires, Centre de Biologie Intégrative, Centre National de la Recherche Scientifique, Université de Toulouse, UPS)

  • Marcelo Nollmann

    (Centre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier)

Abstract

Precise and rapid DNA segregation is required for proper inheritance of genetic material. In most bacteria and archaea, this process is assured by a broadly conserved mitotic-like apparatus in which a NTPase (ParA) displaces the partition complex. Competing observations and models imply starkly different 3D localization patterns of the components of the partition machinery during segregation. Here we use super-resolution microscopies to localize in 3D each component of the segregation apparatus with respect to the bacterial chromosome. We show that Par proteins locate within the nucleoid volume and reveal that proper volumetric localization and segregation of partition complexes requires ATPase and DNA-binding activities of ParA. Finally, we find that the localization patterns of the different components of the partition system highly correlate with dense chromosomal regions. We propose a new mechanism in which the nucleoid provides a scaffold to guide the proper segregation of partition complexes.

Suggested Citation

  • Antoine Le Gall & Diego I. Cattoni & Baptiste Guilhas & Céline Mathieu-Demazière & Laura Oudjedi & Jean-Bernard Fiche & Jérôme Rech & Sara Abrahamsson & Heath Murray & Jean-Yves Bouet & Marcelo Nollma, 2016. "Bacterial partition complexes segregate within the volume of the nucleoid," Nature Communications, Nature, vol. 7(1), pages 1-10, November.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms12107
    DOI: 10.1038/ncomms12107
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    Cited by:

    1. Lavisha Jindal & Eldon Emberly, 2019. "DNA segregation under Par protein control," PLOS ONE, Public Library of Science, vol. 14(7), pages 1-20, July.
    2. Constantin N. Takacs & Jenny Wachter & Yingjie Xiang & Zhongqing Ren & Xheni Karaboja & Molly Scott & Matthew R. Stoner & Irnov Irnov & Nicholas Jannetty & Patricia A. Rosa & Xindan Wang & Christine J, 2022. "Polyploidy, regular patterning of genome copies, and unusual control of DNA partitioning in the Lyme disease spirochete," Nature Communications, Nature, vol. 13(1), pages 1-22, December.

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