IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v7y2016i1d10.1038_ncomms11876.html
   My bibliography  Save this article

Alix-mediated assembly of the actomyosin–tight junction polarity complex preserves epithelial polarity and epithelial barrier

Author

Listed:
  • Yvan Campos

    (St Jude Children’s Research Hospital)

  • Xiaohui Qiu

    (St Jude Children’s Research Hospital)

  • Elida Gomero

    (St Jude Children’s Research Hospital)

  • Randall Wakefield

    (Cellular Imaging Shared Resource, St Jude Children’s Research Hospital)

  • Linda Horner

    (Cellular Imaging Shared Resource, St Jude Children’s Research Hospital)

  • Wojciech Brutkowski

    (Laboratory of Imaging Tissue Structure and Function, Nencki Institute of Experimental Biology, Polish Academy of Sciences)

  • Young-Goo Han

    (St Jude Children’s Research Hospital)

  • David Solecki

    (St Jude Children’s Research Hospital)

  • Sharon Frase

    (Cellular Imaging Shared Resource, St Jude Children’s Research Hospital)

  • Antonella Bongiovanni

    (Institute of Biomedicine and Molecular Immunology, National Research Council)

  • Alessandra d’Azzo

    (St Jude Children’s Research Hospital)

Abstract

Maintenance of epithelial cell polarity and epithelial barrier relies on the spatial organization of the actin cytoskeleton and proper positioning/assembly of intercellular junctions. However, how these processes are regulated is poorly understood. Here we reveal a key role for the multifunctional protein Alix in both processes. In a knockout mouse model of Alix, we identified overt structural changes in the epithelium of the choroid plexus and in the ependyma, such as asymmetrical cell shape and size, misplacement and abnormal beating of cilia, blebbing of the microvilli. These defects culminate in excessive cell extrusion, enlargement of the lateral ventricles and hydrocephalus. Mechanistically, we find that by interacting with F-actin, the Par complex and ZO-1, Alix ensures the formation and maintenance of the apically restricted actomyosin–tight junction complex. We propose that in this capacity Alix plays a role in the establishment of apical–basal polarity and in the maintenance of the epithelial barrier.

Suggested Citation

  • Yvan Campos & Xiaohui Qiu & Elida Gomero & Randall Wakefield & Linda Horner & Wojciech Brutkowski & Young-Goo Han & David Solecki & Sharon Frase & Antonella Bongiovanni & Alessandra d’Azzo, 2016. "Alix-mediated assembly of the actomyosin–tight junction polarity complex preserves epithelial polarity and epithelial barrier," Nature Communications, Nature, vol. 7(1), pages 1-15, September.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11876
    DOI: 10.1038/ncomms11876
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/ncomms11876
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/ncomms11876?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. B. Vijayalakshmi Ayyar & Khalil Ettayebi & Wilhelm Salmen & Umesh C. Karandikar & Frederick H. Neill & Victoria R. Tenge & Sue E. Crawford & Erhard Bieberich & B. V. Venkataram Prasad & Robert L. Atma, 2023. "CLIC and membrane wound repair pathways enable pandemic norovirus entry and infection," Nature Communications, Nature, vol. 14(1), pages 1-14, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11876. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.