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Interphase adhesion geometry is transmitted to an internal regulator for spindle orientation via caveolin-1

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  • Shigeru Matsumura

    (Institute for Virus Research, Kyoto University, Sakyo-ku)

  • Tomoko Kojidani

    (Advanced ICT Research Institute Kobe, National Institute of Information and Communication Technology, Nishi-ku
    Japan Women’s University, Bunkyo-ku)

  • Yuji Kamioka

    (Kyoto University
    Innovative Techno-Hub for Integrated Medical Bio-Imaging, Kyoto University)

  • Seiichi Uchida

    (Faculty of Information Science and Electrical Engineering, Kyushu University, Nishi-ku)

  • Tokuko Haraguchi

    (Advanced ICT Research Institute Kobe, National Institute of Information and Communication Technology, Nishi-ku)

  • Akatsuki Kimura

    (Cell Architecture Laboratory, Structural Biology Center, National Institute of Genetics)

  • Fumiko Toyoshima

    (Institute for Virus Research, Kyoto University, Sakyo-ku)

Abstract

Despite theoretical and physical studies implying that cell-extracellular matrix adhesion geometry governs the orientation of the cell division axis, the molecular mechanisms that translate interphase adhesion geometry to the mitotic spindle orientation remain elusive. Here, we show that the cellular edge retraction during mitotic cell rounding correlates with the spindle axis. At the onset of mitotic cell rounding, caveolin-1 is targeted to the retracting cortical region at the proximal end of retraction fibres, where ganglioside GM1-enriched membrane domains with clusters of caveola-like structures are formed in an integrin and RhoA-dependent manner. Furthermore, Gαi1–LGN–NuMA, a well-known regulatory complex of spindle orientation, is targeted to the caveolin-1-enriched cortical region to guide the spindle axis towards the cellular edge retraction. We propose that retraction-induced cortical heterogeneity of caveolin-1 during mitotic cell rounding sets the spindle orientation in the context of adhesion geometry.

Suggested Citation

  • Shigeru Matsumura & Tomoko Kojidani & Yuji Kamioka & Seiichi Uchida & Tokuko Haraguchi & Akatsuki Kimura & Fumiko Toyoshima, 2016. "Interphase adhesion geometry is transmitted to an internal regulator for spindle orientation via caveolin-1," Nature Communications, Nature, vol. 7(1), pages 1-12, September.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11858
    DOI: 10.1038/ncomms11858
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