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A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging

Author

Listed:
  • Takahiro Kuchimaru

    (School of Life Science and Technology, Tokyo Institute of Technology)

  • Satoshi Iwano

    (Graduate School of Informatics and Engineering, The University of Electro-Communications)

  • Masahiro Kiyama

    (Graduate School of Informatics and Engineering, The University of Electro-Communications)

  • Shun Mitsumata

    (School of Life Science and Technology, Tokyo Institute of Technology)

  • Tetsuya Kadonosono

    (School of Life Science and Technology, Tokyo Institute of Technology)

  • Haruki Niwa

    (Graduate School of Informatics and Engineering, The University of Electro-Communications)

  • Shojiro Maki

    (Graduate School of Informatics and Engineering, The University of Electro-Communications)

  • Shinae Kizaka-Kondoh

    (School of Life Science and Technology, Tokyo Institute of Technology)

Abstract

In preclinical cancer research, bioluminescence imaging with firefly luciferase and D-luciferin has become a standard to monitor biological processes both in vitro and in vivo. However, the emission maximum (λmax) of bioluminescence produced by D-luciferin is 562 nm where light is not highly penetrable in biological tissues. This emphasizes a need for developing a red-shifted bioluminescence imaging system to improve detection sensitivity of targets in deep tissue. Here we characterize the bioluminescent properties of the newly synthesized luciferin analogue, AkaLumine-HCl. The bioluminescence produced by AkaLumine-HCl in reactions with native firefly luciferase is in the near-infrared wavelength ranges (λmax=677 nm), and yields significantly increased target-detection sensitivity from deep tissues with maximal signals attained at very low concentrations, as compared with D-luciferin and emerging synthetic luciferin CycLuc1. These characteristics offer a more sensitive and accurate method for non-invasive bioluminescence imaging with native firefly luciferase in various animal models.

Suggested Citation

  • Takahiro Kuchimaru & Satoshi Iwano & Masahiro Kiyama & Shun Mitsumata & Tetsuya Kadonosono & Haruki Niwa & Shojiro Maki & Shinae Kizaka-Kondoh, 2016. "A luciferin analogue generating near-infrared bioluminescence achieves highly sensitive deep-tissue imaging," Nature Communications, Nature, vol. 7(1), pages 1-8, September.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11856
    DOI: 10.1038/ncomms11856
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    Cited by:

    1. Gregory T. Kennedy & Feredun S. Azari & Elizabeth Bernstein & Bilal Nadeem & Ashley Chang & Alix Segil & Sean Carlin & Neil T. Sullivan & Emmanuel Encarnado & Charuhas Desphande & Sumith Kularatne & P, 2022. "Targeted detection of cancer at the cellular level during biopsy by near-infrared confocal laser endomicroscopy," Nature Communications, Nature, vol. 13(1), pages 1-9, December.

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