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In vivo modulation of endothelial polarization by Apelin receptor signalling

Author

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  • Hyouk-Bum Kwon

    (Max Planck Institute for Heart and Lung Research)

  • Shengpeng Wang

    (Max Planck Institute for Heart and Lung Research)

  • Christian S. M. Helker

    (Max Planck Institute for Heart and Lung Research)

  • S. Javad Rasouli

    (Max Planck Institute for Heart and Lung Research)

  • Hans-Martin Maischein

    (Max Planck Institute for Heart and Lung Research)

  • Stefan Offermanns

    (Max Planck Institute for Heart and Lung Research)

  • Wiebke Herzog

    (University of Münster
    Max Planck Institute for Molecular Biomedicine)

  • Didier Y. R. Stainier

    (Max Planck Institute for Heart and Lung Research)

Abstract

Endothelial cells (ECs) respond to shear stress by aligning in the direction of flow. However, how ECs respond to flow in complex in vivo environments is less clear. Here we describe an endothelial-specific transgenic zebrafish line, whereby the Golgi apparatus is labelled to allow for in vivo analysis of endothelial polarization. We find that most ECs polarize within 4.5 h after the onset of vigorous blood flow and, by manipulating cardiac function, observe that flow-induced EC polarization is a dynamic and reversible process. Based on its role in EC migration, we analyse the role of Apelin signalling in EC polarization and find that it is critical for this process. Knocking down Apelin receptor function in human primary ECs also affects their polarization. Our study provides new tools to analyse the mechanisms of EC polarization in vivo and reveals an important role in this process for a signalling pathway implicated in cardiovascular disease.

Suggested Citation

  • Hyouk-Bum Kwon & Shengpeng Wang & Christian S. M. Helker & S. Javad Rasouli & Hans-Martin Maischein & Stefan Offermanns & Wiebke Herzog & Didier Y. R. Stainier, 2016. "In vivo modulation of endothelial polarization by Apelin receptor signalling," Nature Communications, Nature, vol. 7(1), pages 1-12, September.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11805
    DOI: 10.1038/ncomms11805
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    1. Laetitia Préau & Anna Lischke & Melanie Merkel & Neslihan Oegel & Maria Weissenbruch & Andria Michael & Hongryeol Park & Dietmar Gradl & Christian Kupatt & Ferdinand Noble, 2024. "Parenchymal cues define Vegfa-driven venous angiogenesis by activating a sprouting competent venous endothelial subtype," Nature Communications, Nature, vol. 15(1), pages 1-22, December.
    2. Shinya Yuge & Koichi Nishiyama & Yuichiro Arima & Yasuyuki Hanada & Eri Oguri-Nakamura & Sanshiro Hanada & Tomohiro Ishii & Yuki Wakayama & Urara Hasegawa & Kazuya Tsujita & Ryuji Yokokawa & Takashi M, 2022. "Mechanical loading of intraluminal pressure mediates wound angiogenesis by regulating the TOCA family of F-BAR proteins," Nature Communications, Nature, vol. 13(1), pages 1-25, December.

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