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Aurora-A recruitment and centrosomal maturation are regulated by a Golgi-activated pool of Src during G2

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  • Maria Luisa Barretta

    (Institute of Protein Biochemistry (IBP), National Research Council (CNR))

  • Daniela Spano

    (Institute of Protein Biochemistry (IBP), National Research Council (CNR))

  • Chiara D’Ambrosio

    (Proteomics and Mass Spectrometry Laboratory, Institute for the Animal Production System in the Mediterranean Environment, ISPAAM, National Research Council (CNR))

  • Romina Ines Cervigni

    (Institute of Protein Biochemistry (IBP), National Research Council (CNR)
    Present address: Division of Neuroscience, San Raffaele Scientific Institute and Vita-Salute University, Milan 20132, Italy.)

  • Andrea Scaloni

    (Proteomics and Mass Spectrometry Laboratory, Institute for the Animal Production System in the Mediterranean Environment, ISPAAM, National Research Council (CNR))

  • Daniela Corda

    (Institute of Protein Biochemistry (IBP), National Research Council (CNR))

  • Antonino Colanzi

    (Institute of Protein Biochemistry (IBP), National Research Council (CNR))

Abstract

The Golgi apparatus is composed of stacks of cisternae laterally connected by tubules to form a ribbon-like structure. At the onset of mitosis, the Golgi ribbon is broken down into discrete stacks, which then undergo further fragmentation. This ribbon cleavage is required for G2/M transition, which thus indicates that a ‘Golgi mitotic checkpoint’ couples Golgi inheritance with cell cycle transition. We previously showed that the Golgi-checkpoint regulates the centrosomal recruitment of the mitotic kinase Aurora-A; however, how the Golgi unlinking regulates this recruitment was unknown. Here we show that, in G2, Aurora-A recruitment is promoted by activated Src at the Golgi. Our data provide evidence that Src and Aurora-A interact upon Golgi ribbon fragmentation; Src phosphorylates Aurora-A at tyrosine 148 and this specific phosphorylation is required for Aurora-A localization at the centrosomes. This process, pivotal for centrosome maturation, is a fundamental prerequisite for proper spindle formation and chromosome segregation.

Suggested Citation

  • Maria Luisa Barretta & Daniela Spano & Chiara D’Ambrosio & Romina Ines Cervigni & Andrea Scaloni & Daniela Corda & Antonino Colanzi, 2016. "Aurora-A recruitment and centrosomal maturation are regulated by a Golgi-activated pool of Src during G2," Nature Communications, Nature, vol. 7(1), pages 1-13, September.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11727
    DOI: 10.1038/ncomms11727
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